Aspects of tetrazolium salt reduction relevant to quantitative histochemistry

Eadie M.J., Tyrer J.H., Kukums J.R. and Hooper W.D. (1970) Aspects of tetrazolium salt reduction relevant to quantitative histochemistry. Histochemie, 21 2: 170-180. doi:10.1007/BF00306184

Author Eadie M.J.
Tyrer J.H.
Kukums J.R.
Hooper W.D.
Title Aspects of tetrazolium salt reduction relevant to quantitative histochemistry
Journal name Histochemie   Check publisher's open access policy
ISSN 0948-6143
Publication date 1970
Sub-type Article (original research)
DOI 10.1007/BF00306184
Volume 21
Issue 2
Start page 170
End page 180
Total pages 11
Publisher Springer-Verlag
Subject 3105 Instrumentation
1307 Cell Biology
2700 Medicine
Abstract Uncertainty about the nature of the reduction products of ditetrazolium salts may have limited their use in quantitative histochemistry. Our studies have shown that under appropriate conditions pure Nitro-BT reduces through a red intermediate substance to a stable blue diformazan. Nitrobenzene was found to be a satisfactory solvent for this diformazan. The monotetrazolium INT may also be reduced to a formazan through an intermediate phase. The amounts of definitive formazan produced from both monotetrazolium and ditetrazolium salts may be influenced by the solubility of their intermediate reduction compounds in the systems in which reduction is occurring. The yield of definitive diformazan from Nitro-BT after chemical reduction, and after enzymatic reduction in liver homogenate and sections of a "mock" tissue, was not in linear proportion to the strength of reducing conditions; however, the yields of formazan from the monotetrazoliums INT and MTT were linear. This finding suggests that in quantitative histochemistry it is essential to calibrate reactions involving ditetrazolium reduction.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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Created: Tue, 14 Jun 2016, 00:48:22 EST by System User