The species of Eimeria in cattle were identified for the first time in Australia. They were E. alabamensis, E. auburnensis, E. bovis, E. brasiliensis, E. brucei, E. bukidnonensis, E. canadensis, E. cylindrica, E. ellipsoidallis, E. hammondi, E. marquardti, E. smithi, E. subspherica, E. wilsoni, and E. zurnii. Of these, E. brucei, E. hammondi, E.marquardti, and E. wilsoni are described as new species.
Sporulation was found to occur in five stages i.e. (1) contraction of the proptoplasmic mass into a sphere, (2) division of the spherical protoplasm into four sporoblasts, (3) separation of the sporoblasts from the central mass, (4) elongation of the sporoblasts, and (5) formation of the sporozoites.
For critical identification a nd differentiation of the species, the sporulation process (at 22°C) of the oocysts was used for the first time, in addition to the general morphology of the oocysts and the sporooysts, and the sporulation time at 22°C. Three distinct types of morphological changes were found to take place during the sporulation process: refractile globules were absent, or they were present and varied in appearance and disposition. Polar globules were found to be common during sporulation but in most species disappeared before the oocysts had sporulated. Extra-protoplasmio globules, a crescent-shaped refractile structure and a refractile projection were characteristic of E. bukidnonensis. of E. smithi, and of E. cylindrica and E. ellipsoidallis respectively.
The endogenous stages of E. alabamensis, E. auburnensis, E. bovis, E. bukidnonensis, E. canadensis, E. cylindrica, E. ellipsoidallis, E. smithi, E. wilsoni, and E. zurnii were studied. Calves were experimentally infected and were destroyed on the day the oocysts were first shed. ..........................