Mapping nano-scale mechanical heterogeneity of primary plant cell walls

Yakubov, Gleb E., Bonilla, Mauricio R., Chen, Huaying, Doblin, Monika S., Bacic, Antony, Gidley, Michael J. and Stokes, Jason R. (2016) Mapping nano-scale mechanical heterogeneity of primary plant cell walls. Journal of Experimental Botany, 67 9: 2799-2816. doi:10.1093/jxb/erw117

Author Yakubov, Gleb E.
Bonilla, Mauricio R.
Chen, Huaying
Doblin, Monika S.
Bacic, Antony
Gidley, Michael J.
Stokes, Jason R.
Title Mapping nano-scale mechanical heterogeneity of primary plant cell walls
Journal name Journal of Experimental Botany   Check publisher's open access policy
ISSN 0022-0957
Publication date 2016
Sub-type Article (original research)
DOI 10.1093/jxb/erw117
Open Access Status DOI
Volume 67
Issue 9
Start page 2799
End page 2816
Total pages 18
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2017
Language eng
Formatted abstract
Nanoindentation experiments are performed using an atomic force microscope (AFM) to quantify the spatial distribution
of mechanical properties of plant cell walls at nanometre length scales. At any specific location on the cell
wall, a complex (non-linear) force–indentation response occurs that can be deconvoluted using a unique multiregime
analysis (MRA). This allows an unambiguous evaluation of the local transverse elastic modulus of the wall.
Nanomechanical measurements on suspension-cultured cells (SCCs), derived from Italian ryegrass (Lolium multiflorum)
starchy endosperm, show three characteristic modes of deformation and a spatial distribution of elastic moduli
across the surface. ‘Soft’ and ‘hard’ domains are found across length scales between 0.1 µm and 3 µm, which is well
above a typical pore size of the polysaccharide mesh. The generality and wider applicability of this mechanical heterogeneity
is verified through in planta characterization on leaf epidermal cells of Arabidopsis thaliana and L. multiflorum.
The outcomes of this research provide a basis for uncovering and quantifying the relationships between local
wall composition, architecture, cell growth, and/or morphogenesis.
Keyword atomic force microscopy
cell mechanics
Lolium multiflorum
primary cell walls
suspended culture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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Created: Tue, 07 Jun 2016, 20:30:09 EST by Gleb Yakubov on behalf of School of Chemical Engineering