Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr-/- mice

Pollock, Abigail H., Tedla, Nicodemus, Hancock, Sarah E., Cornely, Rhea, Mitchell, Todd W., Yang, Zhengmin, Kockx, Maaike, Parton, Robert G., Rossy, Jeremie and Gaus, Katharina (2016) Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr-/- mice. Journal of Immunology, 196 10: 3993-4002. doi:10.4049/jimmunol.1501261


Author Pollock, Abigail H.
Tedla, Nicodemus
Hancock, Sarah E.
Cornely, Rhea
Mitchell, Todd W.
Yang, Zhengmin
Kockx, Maaike
Parton, Robert G.
Rossy, Jeremie
Gaus, Katharina
Title Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr-/- mice
Formatted title
Prolonged intake of dietary lipids alters membrane structure and T cell responses in LDLr-/- mice
Journal name Journal of Immunology   Check publisher's open access policy
ISSN 0022-1767
1550-6606
Publication date 2016-05
Year available 2016
Sub-type Article (original research)
DOI 10.4049/jimmunol.1501261
Open Access Status Not Open Access
Volume 196
Issue 10
Start page 3993
End page 4002
Total pages 10
Place of publication Bethesda, MD, United States
Publisher American Association of Immunologists
Collection year 2017
Language eng
Formatted abstract
Although it is recognized that lipids and membrane organization in T cells affect signaling and T cell activation, to what extent dietary lipids alter T cell responsiveness in the absence of obesity and inflammation is not known. In this study, we fed low-density lipoprotein receptor knockout mice a Western high-fat diet for 1 or 9 wk and examined T cell responses in vivo along with T cell lipid composition, membrane order, and activation ex vivo. Our data showed that high levels of circulating lipids for a prolonged period elevated CD4+ and CD8+ T cell proliferation and resulted in an increased proportion of CD4+ central-memory T cells within the draining lymph nodes following induction of contact hypersensitivity. In addition, the 9-wk Western high-fat diet elevated the total phospholipid content and monounsaturated fatty acid level, but decreased saturated phosphatidylcholine and sphingomyelin within the T cells. The altered lipid composition in the circulation, and of T cells, was also reflected by enhanced membrane order at the activation site of ex vivo activated T cells that corresponded to increased IL-2 mRNA levels. In conclusion, dietary lipids can modulate T cell lipid composition and responses in lipoprotein receptor knockout mice even in the absence of excess weight gain and a proinflammatory environment.
Keyword Low-density lipoprotein receptor
Plasma membrane
Receptor knockout
Dietary lipids
Membrane structure
T cell responses
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
Centre for Microscopy and Microanalysis Publications
 
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