Effects of long-term tenofovir-based cART in HIV-HBV co-infection on persistent HBV viremia and the role of HBV quasispecies diversity

Audsley, Jennifer, Bent, Stephen J., Littlejohn, Margaret, Avihingsanon, Anchalee, Matthews, Gail, Bowden, Scott, Bayliss, Julianne, Luciani, Fabio, Yuen, Lilly, Fairley, Christopher K., Locarnini, Stephen, Lewin, Sharon R. and Sasadeusz, Joe (2016) Effects of long-term tenofovir-based cART in HIV-HBV co-infection on persistent HBV viremia and the role of HBV quasispecies diversity. AIDS, 30 10: 1597-1606. doi:10.1097/QAD.0000000000001080


Author Audsley, Jennifer
Bent, Stephen J.
Littlejohn, Margaret
Avihingsanon, Anchalee
Matthews, Gail
Bowden, Scott
Bayliss, Julianne
Luciani, Fabio
Yuen, Lilly
Fairley, Christopher K.
Locarnini, Stephen
Lewin, Sharon R.
Sasadeusz, Joe
Title Effects of long-term tenofovir-based cART in HIV-HBV co-infection on persistent HBV viremia and the role of HBV quasispecies diversity
Journal name AIDS   Check publisher's open access policy
ISSN 1473-5571
0269-9370
Publication date 2016-06-19
Year available 2016
Sub-type Article (original research)
DOI 10.1097/QAD.0000000000001080
Open Access Status Not Open Access
Volume 30
Issue 10
Start page 1597
End page 1606
Total pages 10
Place of publication Philadelphia, PA United States
Publisher Lippincott Williams & Wilkins
Collection year 2017
Language eng
Formatted abstract
Objective: Hepatitis B virus (HBV) can persist in some HIV–HBV coinfected individuals on tenofovir disoproxil fumarate (TDF)-containing combination antiretroviral therapy (cART) but HBV resistance to TDF has not been reported and the source of persistent HBV DNA on TDF is poorly understood. The aims of this study were to assess long-term HBV suppression in HIV–HBV coinfected individuals receiving TDF and investigate quasispecies variation using ultradeep pyrosequencing (UDPS).

Methods: Ninety-two HIV–HBV coinfected participants on, or about to commence, TDF-containing cART were enrolled [Australia (n = 40), Thailand (n = 52)] and followed for 2 years with study visits every 6 months. HBV reverse transcriptase sequencing was performed on samples with HBV DNA more than 400 IU/ml by population-based methods and UDPS. Quasispecies diversity was assessed using Shannon entropy.

Results: Over 24 months, viremia was detected at least once in 17% (n = 16) of the cohort. Novel mutations were not identified in on TDF samples tested by population-based sequencing (n = 19). Using UDPS, the median Shannon entropy value in samples prior to TDF in patients aviremic on TDF was not statistically different from those who were viremic on TDF (n = 50; 8.4 and 9.1, respectively, P = 0.9). Longitudinal Shannon entropy analysis of on TDF samples from five participants showed three individuals with significant changes in viral diversity over time.

Conclusion: Persistent viremia on TDF-containing cART is common but TDF-resistance was not detected. In some individuals, changes in viral diversity over time were observed on TDF which could potentially be active viral replication. Further follow-up will be needed to determine the clinical significance of detectable HBV DNA on TDF-containing cART.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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Created: Tue, 31 May 2016, 11:17:31 EST by Stephen Bent on behalf of Institute for Molecular Bioscience