Quantitative PCR for detection of babesia microti in ixodes scapularis ticks and in human blood

Rollend, Lindsay, Bent, Stephen J., Krause, Peter J., Usmani-Brown, Sahar, Steeves, Tanner K., States, Sarah L., Lepore, Timothy, Ryan, Raymond, Dias, Fil, Ben Mamoun, Choukri, Fish, Durland and Diuk-Wasser, Maria A. (2013) Quantitative PCR for detection of babesia microti in ixodes scapularis ticks and in human blood. Vector-Borne and Zoonotic Diseases, 13 11: 784-790. doi:10.1089/vbz.2011.0935


Author Rollend, Lindsay
Bent, Stephen J.
Krause, Peter J.
Usmani-Brown, Sahar
Steeves, Tanner K.
States, Sarah L.
Lepore, Timothy
Ryan, Raymond
Dias, Fil
Ben Mamoun, Choukri
Fish, Durland
Diuk-Wasser, Maria A.
Title Quantitative PCR for detection of babesia microti in ixodes scapularis ticks and in human blood
Formatted title
Quantitative PCR for detection of babesia microti in ixodes scapularis ticks and in human blood
Journal name Vector-Borne and Zoonotic Diseases   Check publisher's open access policy
ISSN 1557-7759
1530-3667
Publication date 2013-11-08
Year available 2013
Sub-type Article (original research)
DOI 10.1089/vbz.2011.0935
Open Access Status Not Open Access
Volume 13
Issue 11
Start page 784
End page 790
Total pages 7
Place of publication New Rochelle, NY, United States
Publisher Mary Ann Liebert
Language eng
Formatted abstract
Babesia microti, the primary cause of human babesiosis in the United States, is transmitted by Ixodes scapularis ticks; transmission may also occur through blood transfusion and transplacentally. Most infected people experience a viral-like illness that resolves without complication, but those who are immunocompromised may develop a serious and prolonged illness that is sometimes fatal. The geographic expansion and increasing incidence of human babesiosis in the northeastern and midwestern United States highlight the need for high-throughput sensitive and specific assays to detect parasites in both ticks and humans with the goals of improving epidemiological surveillance, diagnosis of acute infections, and screening of the blood supply. Accordingly, we developed a B. microti-specific quantitative PCR (qPCR) assay (named BabMq18) designed to detect B. microti DNA in tick and human blood samples using a primer and probe combination that targets the 18S rRNA gene of B. microti. This qPCR assay was compared with two nonquantitative B. microti PCR assays by testing tick samples and was found to exhibit higher sensitivity for detection of B. microti DNA. The BabMq18 assay has a detection threshold of 10 copies per reaction and does not amplify DNA in I. scapularis ticks infected with Babesia odocoilei, Borrelia burgdorferi, Borrelia miyamotoi, or Anaplasma phagocytophilum. This highly sensitive and specific qPCR assay can be used for detection of B. microti DNA in both tick and human samples. Finally, we report the prevalence of B. microti infection in field-collected I. scapularis nymphs from three locations in southern New England that present disparate incidences of human babesiosis.
Keyword Babesia microti
Babesia odocoilei
Babesiosis
Ixodes scapularis
Quantitative PCR
Ticks
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biological Sciences Publications
 
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Created: Tue, 31 May 2016, 11:11:03 EST by Stephen Bent on behalf of Learning and Research Services (UQ Library)