Spatial distribution of Rhodopseudomonas palustris ecotypes on a local scale

Bent, S. J., Gucker, C. L., Oda, Y. and Forney, L. J. (2003) Spatial distribution of Rhodopseudomonas palustris ecotypes on a local scale. Applied and Environmental Microbiology, 69 9: 5192-5197. doi:10.1128/AEM.69.9.5192-5197.2003

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Author Bent, S. J.
Gucker, C. L.
Oda, Y.
Forney, L. J.
Title Spatial distribution of Rhodopseudomonas palustris ecotypes on a local scale
Formatted title
Spatial distribution of Rhodopseudomonas palustris ecotypes on a local scale
Journal name Applied and Environmental Microbiology   Check publisher's open access policy
ISSN 0099-2240
Publication date 2003-09
Sub-type Article (original research)
DOI 10.1128/AEM.69.9.5192-5197.2003
Open Access Status File (Publisher version)
Volume 69
Issue 9
Start page 5192
End page 5197
Total pages 6
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The number, spatial distribution, and significance of genetically distinguishable ecotypes of prokaryotes in the environment are poorly understood. Oda et al. (Y. Oda, B. Star, L. A. Huisman, J. C. Gottschal, and L. J. Forney, Appl. Environ. Microbiol. 69:xxx-xxx, 2003) have shown that Rhodopseudomonas palustris ecotypes were lognormally distributed along a 10-m transect and that multiple strains of the species could coexist in 0.5-g sediment samples. To extend these observations, we investigated the clonal diversity of R. palustris in 0.5-g samples taken from the corners and center of a 1-m square. A total of 35 or 36 clones were recovered by direct plating from each sample and were characterized by BOX A1R repetitive element-PCR genomic DNA fingerprinting. Isolates with fingerprint images that were ≥80% similar to each other were defined as the same genotype. Among the 178 isolates studied, 32 genotypes were identified, and each genotype contained between 1 and 40 isolates. These clusters were consistent with minor variations found in 16S rRNA gene sequences. The Shannon indices of the genotypic diversity within each location ranged from 1.08 (5 genotypes) to 2.18 (13 genotypes). Comparison of the rank abundance of genotypes found in pairs of locations showed that strains from three locations were similar to each other, with Morisita-Horn similarity coefficients ranging from 0.59 to 0.71. All comparisons involving the remaining two locations resulted in coefficients between 0 and 0.12. From these results we inferred that the patterns of ecotype diversity at the sampling site are patchy at a 1-m scale and postulated that factors such as mixing, competitive interactions, and microhabitat variability are likely to be responsible for the maintenance of the similarities between some locations and the differences between others.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
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Citation counts: TR Web of Science Citation Count  Cited 23 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 31 May 2016, 11:01:09 EST by Stephen Bent on behalf of Learning and Research Services (UQ Library)