Parturition in dairy cows temporarily alters the expression of genes in circulating neutrophils

Crookenden, M. A., Heiser, A., Murray, A., Dukkipati, V. S. R., Kay, J. K., Loor, J. J., Meier, S., Mitchell, M. D., Moyes, K. M., Walker, C. G. and Roche, J. R. (2016) Parturition in dairy cows temporarily alters the expression of genes in circulating neutrophils. Journal of Dairy Science, 99 8: 6470-6483. doi:10.3168/jds.2015-10877


Author Crookenden, M. A.
Heiser, A.
Murray, A.
Dukkipati, V. S. R.
Kay, J. K.
Loor, J. J.
Meier, S.
Mitchell, M. D.
Moyes, K. M.
Walker, C. G.
Roche, J. R.
Title Parturition in dairy cows temporarily alters the expression of genes in circulating neutrophils
Journal name Journal of Dairy Science   Check publisher's open access policy
ISSN 1525-3198
0022-0302
Publication date 2016-08
Year available 2016
Sub-type Article (original research)
DOI 10.3168/jds.2015-10877
Open Access Status DOI
Volume 99
Issue 8
Start page 6470
End page 6483
Total pages 14
Place of publication New York, NY, United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Extensive metabolic and physiologic changes occur during the peripartum, concurrent with a high incidence of infectious disease. Immune dysfunction is a likely contributor to the increased risk of disease at this time. Studies using high-yielding, total mixed ration-fed cows have indicated that neutrophil function is perturbed over the transition period; however, this reported dysfunction has yet to be investigated in moderate-yielding, grazing dairy cows. Therefore, we investigated changes in the expression of genes involved in neutrophil function. Blood was collected from cows at 5 time points over the transition period: precalving (-1 wk; n = 46), day of calving (d 0; n = 46), and postcalving at wk 1 (n = 46), wk 2 (n = 45), and wk 4 (n = 43). Neutrophils were isolated by differential centrifugation and gene expression was investigated. Quantitative reverse transcriptase PCR with custom-designed primer pairs and Roche Universal Probe Library (Roche, Basel, Switzerland) chemistry, combined with microfluidics integrated fluidic circuit chips (96.96 Dynamic Array, San Francisco, CA) were used to investigate the expression of 78 genes involved in neutrophil function and 18 endogenous control genes. Statistical significance between time points was determined using a repeated measures ANOVA. Genes that were differentially expressed over the transition period included those involved in neutrophil adhesion (SELL, ITGB2, and ITGBX), mediation of the immune response (TLR4, HLA-DRA, and CXCR2), maturation, cell cycle progression, apoptosis (MCL1, BCL2, FASLG, and RIPK1), and control of gene expression (PPARG, PPARD, and STAT3). We noted reduced gene expression of proinflammatory cytokines (IFNG, TNF, IL12, and CCL2) on the day of calving, whereas anti-inflammatory cytokine gene expression (IL10) was upregulated. Increased gene expression of antimicrobial peptides (BNBD4, DEFB10, and DEFB1) occurred on the day of calving. Collectively, transcription profiles are indicative of functional changes in neutrophils of grazing dairy cows over the transition period and align with studies in cows of conventional total mixed ration systems. This altered function may predispose cows to disease over the transition period and is likely to be a natural change in function due to parturition
Keyword Fluidigm
Innate immune function
Peripartum period
PMN
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
HERDC Pre-Audit
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 24 May 2016, 03:54:36 EST by System User on behalf of Learning and Research Services (UQ Library)