Evaluation of equine peripheral blood apheresis product, bone marrow, and adipose tissue as sources of mesenchymal stem cells and their differentation potential

Ahern, Benjamin J., Schaer, Thomas P., Terkhorn, Shawn P., Jackson, Karen V., Mason, Nicola J. and Hankenson, Kurt D. (2011) Evaluation of equine peripheral blood apheresis product, bone marrow, and adipose tissue as sources of mesenchymal stem cells and their differentation potential. American Journal of Veterinary Research, 72 1: 127-133. doi:10.2460/ajvr.72.1.127


Author Ahern, Benjamin J.
Schaer, Thomas P.
Terkhorn, Shawn P.
Jackson, Karen V.
Mason, Nicola J.
Hankenson, Kurt D.
Title Evaluation of equine peripheral blood apheresis product, bone marrow, and adipose tissue as sources of mesenchymal stem cells and their differentation potential
Journal name American Journal of Veterinary Research   Check publisher's open access policy
ISSN 0002-9645
1943-5681
Publication date 2011-01
Sub-type Article (original research)
DOI 10.2460/ajvr.72.1.127
Open Access Status Not Open Access
Volume 72
Issue 1
Start page 127
End page 133
Total pages 7
Place of publication Schaumburg, IL United States
Publisher American Veterinary Medical Association
Language eng
Formatted abstract
Objective—To evaluate effects of apheresis on mesenchymal stem cells (MSCs) and compare those MSCs with MSCs obtained from adipose tissue or bone marrow (BM).

Sample Population—Samples obtained from 6 adult horses.

Procedures—Samples of blood from a peripheral vein, adipose tissue, and BM aspirate were obtained from each horse. Samples were processed via apheresis of blood and techniques reported elsewhere for adipose tissue and BM. Cultures were maintained until adherence and subsequently were subjected to differentiation protocols to evaluate adipogenic, osteoblastogenic, and chondrogenic potential.

Results—Apheresis product had a significantly higher mononuclear percentage, higher platelet count, and lower RBC count, compared with values for peripheral blood. No cell adherence to the tissue culture plates was detected for the apheresis product. Adherence was detected for 6 of 6 adipose-derived and 4 of 6 BM-derived samples. Variations in efficiency were detected for differentiation of adipose- and BM-derived cells into adipocytes, chondrocytes, and osteoblasts.

Conclusions and Clinical Relevance—Apheresis was able to concentrate mononuclear cells and reduce RBC contamination. However, the apheresis product was unable to adhere to the tissue culture plates. In matched horses, adipose- and BM-derived MSCs were capable of producing lipids, glycosaminoglycan, and mineral. The BM was vastly superior to adipose tissue as a source of MSCs with osteoblastogenic potential in matched horses. Additional studies will be necessary to optimize apheresis techniques for horses before peripheral blood can be considered a suitable source for multipotential cells for use in cell-based treatments.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Tue, 17 May 2016, 14:53:42 EST by Karen Jackson on behalf of School of Veterinary Science