Comparison of gel column, card, and cartridge techniques for dog erythrocyte antigen 1.1 blood typing

Seth, Mayank, Jackson, Karen V., Winzelberg, Sarah and Giger, Urs (2012) Comparison of gel column, card, and cartridge techniques for dog erythrocyte antigen 1.1 blood typing. American Journal of Veterinary Research, 73 2: 213-219. doi:10.2460/ajvr.73.2.213

Author Seth, Mayank
Jackson, Karen V.
Winzelberg, Sarah
Giger, Urs
Title Comparison of gel column, card, and cartridge techniques for dog erythrocyte antigen 1.1 blood typing
Journal name American Journal of Veterinary Research   Check publisher's open access policy
ISSN 0002-9645
Publication date 2012-02
Sub-type Article (original research)
DOI 10.2460/ajvr.73.2.213
Open Access Status Not Open Access
Volume 73
Issue 2
Start page 213
End page 219
Total pages 7
Place of publication Schaumburg, IL United States
Publisher American Veterinary Medical Association
Language eng
Formatted abstract
Objective—To compare accuracy and ease of use of a card agglutination assay, an immunochromatographic cartridge method, and a gel-based method for canine blood typing.

Sample—Blood samples from 52 healthy blood donor dogs, 10 dogs with immune-mediated hemolytic anemia (IMHA), and 29 dogs with other diseases.

Procedures—Blood samples were tested in accordance with manufacturer guidelines. Samples with low PCVs were created by the addition of autologous plasma to separately assess the effects of anemia on test results.

Results—Compared with a composite reference standard of agreement between 2 methods, the gel-based method was found to be 100% accurate. The card agglutination assay was 89% to 91% accurate, depending on test interpretation, and the immunochromatographic cartridge method was 93% accurate but 100% specific. Errors were observed more frequently in samples from diseased dogs, particularly those with IMHA. In the presence of persistent autoagglutination, dog erythrocyte antigen (DEA) 1.1 typing was not possible, except with the immunochromatographic cartridge method.

Conclusions and Clinical Relevance—The card agglutination assay and immunochromatographic cartridge method, performed by trained personnel, were suitable for in-clinic emergency DEA 1.1 blood typing. There may be errors, particularly for samples from dogs with IMHA, and the immunochromatographic cartridge method may have an advantage of allowing typing of samples with persistent autoagglutination. The laboratory gel-based method would be preferred for routine DEA 1.1 typing of donors and patients if it is available and time permits. Current DEA 1.1 typing techniques appear to be appropriately standardized and easy to use.
Keyword Transfusion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
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Created: Tue, 17 May 2016, 14:52:21 EST by Karen Jackson on behalf of School of Veterinary Science