Genetic conservation of phosphine resistance in the rice weevil Sitophilus oryzae (L.)

Nguyen, Tam T., Collins, Patrick J., Duong, Tu M., Schlipalius, David I. and Ebert, Paul R. (2016) Genetic conservation of phosphine resistance in the rice weevil Sitophilus oryzae (L.). Journal of Heredity, 107 3: 228-237. doi:10.1093/jhered/esw001


Author Nguyen, Tam T.
Collins, Patrick J.
Duong, Tu M.
Schlipalius, David I.
Ebert, Paul R.
Title Genetic conservation of phosphine resistance in the rice weevil Sitophilus oryzae (L.)
Formatted title
Genetic conservation of phosphine resistance in the rice weevil Sitophilus oryzae (L.)
Journal name Journal of Heredity   Check publisher's open access policy
ISSN 1465-7333
0022-1503
Publication date 2016-05-01
Year available 2016
Sub-type Article (original research)
DOI 10.1093/jhered/esw001
Open Access Status Not Open Access
Volume 107
Issue 3
Start page 228
End page 237
Total pages 10
Place of publication Oxford, United Kingdom
Publisher Oxford University Press
Collection year 2017
Language eng
Formatted abstract
High levels of resistance to phosphine in the rice weevil Sitophilus oryzae have been detected in Asian countries including China and Vietnam, however there is limited knowledge of the genetic mechanism of resistance in these strains. We find that the genetic basis of strong phosphine resistance is conserved between strains of S. oryzae from China, Vietnam, and Australia. Each of 4 strongly resistant strains has an identical amino acid variant in the encoded dihydrolipoamide dehydrogenase (DLD) enzyme that was previously identified as a resistance factor in Rhyzopertha dominica and Tribolium castaneum. The unique amino acid substitution, Asparagine > Threonine (N505T) of all strongly resistant S. oryzae corresponds to the position of an Asparagine > Histidine variant (N506H) that was previously reported in strongly resistant R. dominica. Progeny (F16 and F18) from 2 independent crosses showed absolute linkage of N505T to the strong resistance phenotype, indicating that if N505T was not itself the resistance variant that it resided within 1 or 2 genes of the resistance factor. Non-complementation between the strains confirmed the shared genetic basis of strong resistance, which was supported by the very similar level of resistance between the strains, with LC50 values ranging from 0.20 to 0.36 mg L-1 for a 48-h exposure at 25 °C. Thus, the mechanism of high-level resistance to phosphine is strongly conserved between R. dominica, T. castaneum and S. oryzae. A fitness cost associated with strongly resistant allele was observed in segregating populations in the absence of selection.
Keyword Dihydrolipoamide dehydrogenase
Fitness cost
Mode of inheritance
Rph2 gene
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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