Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation

Brambilla, Eugenio, Ionescu, Andrew C., Cazzaniga, Gloria, Ottobelli, Marco and Samaranayake, Lakshman P. (2016) Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation. Journal of Basic Microbiology, 56 5: 480-492. doi:10.1002/jobm.201500329


Author Brambilla, Eugenio
Ionescu, Andrew C.
Cazzaniga, Gloria
Ottobelli, Marco
Samaranayake, Lakshman P.
Title Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation
Formatted title
Levorotatory carbohydrates and xylitol subdue Streptococcus mutans and Candida albicans adhesion and biofilm formation
Journal name Journal of Basic Microbiology   Check publisher's open access policy
ISSN 1521-4028
Publication date 2016-05-01
Year available 2016
Sub-type Article (original research)
DOI 10.1002/jobm.201500329
Open Access Status Not Open Access
Volume 56
Issue 5
Start page 480
End page 492
Total pages 13
Place of publication Weinheim, Germany
Publisher Wiley-VCH Verlag
Collection year 2017
Language eng
Formatted abstract
Dietary carbohydrates and polyols affect the microbial colonization of oral surfaces by modulating adhesion and biofilm formation. The aim of this study was to evaluate the influence of a select group of l-carbohydrates and polyols on either Streptococcus mutans or Candida albicans adhesion and biofilm formation in vitro. S. mutans or C. albicans suspensions were inoculated on polystyrene substrata in the presence of Tryptic soy broth containing 5% of the following compounds: d-glucose, d-mannose, l-glucose, l-mannose, d- and l-glucose (raceme), d- and l-mannose (raceme), l-glucose and l-mannose, sorbitol, mannitol, and xylitol. Microbial adhesion (2 h) and biofilm formation (24 h) were evaluated using MTT-test and Scanning Electron Microscopy (SEM). Xylitol and l-carbohydrates induced the lowest adhesion and biofilm formation in both the tested species, while sorbitol and mannitol did not promote C. albicans biofilm formation. Higher adhesion and biofilm formation was noted in both organisms in the presence of d-carbohydrates relative to their l-carbohydrate counterparts. These results elucidate, hitherto undescribed, interactions of the individually tested strains with l- and d-carbohydrates, and how they impact fungal and bacterial colonization. In translational terms, our data raise the possibility of using l-form of carbohydrates and xylitol for dietary control of oral plaque biofilms.
Keyword Levorotatory carbohydrate
Xylitol
Streptococcus mutans
Candida albicans
Biofilm
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Dentistry Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 17 May 2016, 10:30:25 EST by System User on behalf of School of Dentistry