Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk

Khalesi, Mohammad, Waterhouse, Mary, Whiteman, David C., Johns, Richard, Rosendahl, Cliff, Hackett, Timothy, Pollak, Thomas, Kimlin, Michael G., Hacker, Elke and Neale, Rachel E. (2016) Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk. International Journal of Dermatology, 55 10: 1096-1105. doi:10.1111/ijd.13276


Author Khalesi, Mohammad
Waterhouse, Mary
Whiteman, David C.
Johns, Richard
Rosendahl, Cliff
Hackett, Timothy
Pollak, Thomas
Kimlin, Michael G.
Hacker, Elke
Neale, Rachel E.
Title Comparison of PTCH1, COX-2, p53, and Ki-67 protein expression in basal cell carcinomas of nodular and superficial subtypes arising on the head and trunk
Journal name International Journal of Dermatology   Check publisher's open access policy
ISSN 1365-4632
0011-9059
Publication date 2016-04-28
Year available 2016
Sub-type Article (original research)
DOI 10.1111/ijd.13276
Open Access Status Not Open Access
Volume 55
Issue 10
Start page 1096
End page 1105
Total pages 10
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Collection year 2017
Language eng
Formatted abstract
Background: There is some evidence that basal cell carcinomas (BCCs) arising on different anatomic sites and developing to different histological subtypes differ in their pathophysiology. The expression of a number of proteins, including PTCH1, COX-2, p53, and Ki-67, is frequently altered in BCC development.

Objectives: This study sought to determine whether protein expression differs between BCCs at different anatomic sites and of different histological subtypes.

Methods: Expression of PTCH1, COX-2, p53, and Ki-67 proteins was compared between: (i) BCCs arising on the head (n = 55) and trunk (n = 53), and (ii) nodular (n = 52) and superficial (n = 43) BCCs. The intensity of immunohistochemistry (IHC) staining (low, moderate, strong, very strong) for PTCH1 and COX-2 proteins was measured and the proportions of p53- and Ki-67-positive cells quantified.

Results: The proportion of cells expressing Ki-67 was higher in tumor tissue than in non-malignant epidermis, whereas the opposite was found for PTCH1. The IHC staining intensity for PTCH1 was substantially greater in truncal BCCs than in BCCs on the head (odds ratio [OR] 3.82, 95% confidence interval [CI] 1.63-8.96). The intensity of staining for PTCH1 was greater for superficial than for nodular BCCs (OR 3.70, 95% CI 1.53-8.97), and superficial BCCs showed a higher proportion of Ki-67-positive cells (OR 5.57, 95% CI 1.66-18.67).

Conclusions: These differences suggest that the pathophysiology of BCC differs between lesions on the head and trunk and between nodular and superficial subtypes, perhaps indicating differences in their etiology.
Keyword Basal cell carcinomas
BCCs
PTCH1
COX-2
Proteins
Lesions
Pathophysiology
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
Admin Only - School of Medicine
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 10 May 2016, 03:06:57 EST by System User on behalf of Learning and Research Services (UQ Library)