Human immune cell targeting of protein nanoparticles-caveospheres

Glass, Joshua J., Yuen, Daniel, Rae, James, Johnston, Angus P. R., Parton, Robert G., Kent, Stephen J. and De Rose, Robert (2016) Human immune cell targeting of protein nanoparticles-caveospheres. Nanoscale, 8 15: 8255-8265. doi:10.1039/c6nr00506c


Author Glass, Joshua J.
Yuen, Daniel
Rae, James
Johnston, Angus P. R.
Parton, Robert G.
Kent, Stephen J.
De Rose, Robert
Title Human immune cell targeting of protein nanoparticles-caveospheres
Journal name Nanoscale   Check publisher's open access policy
ISSN 2040-3372
2040-3364
Publication date 2016-04-21
Year available 2016
Sub-type Article (original research)
DOI 10.1039/c6nr00506c
Open Access Status Not Open Access
Volume 8
Issue 15
Start page 8255
End page 8265
Total pages 11
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Collection year 2017
Language eng
Abstract Nanotechnology has the power to transform vaccine and drug delivery through protection of payloads from both metabolism and off-target effects, while facilitating specific delivery of cargo to immune cells. However, evaluation of immune cell nanoparticle targeting is conventionally restricted to monocultured cell line models. We generated human caveolin-1 nanoparticles, termed caveospheres, which were efficiently functionalized with monoclonal antibodies. Using this platform, we investigated CD4+ T cell and CD20+ B cell targeting within physiological mixtures of primary human blood immune cells using flow cytometry, imaging flow cytometry and confocal microscopy. Antibody-functionalization enhanced caveosphere binding to targeted immune cells (6.6 to 43.9-fold) within mixed populations and in the presence of protein-containing fluids. Moreover, targeting caveospheres to CCR5 enabled caveosphere internalization by non-phagocytic CD4+ T cells - an important therapeutic target for HIV treatment. This efficient and flexible system of immune cell-targeted caveosphere nanoparticles holds promise for the development of advanced immunotherapeutics and vaccines.
Keyword Human immune cell
Nanoparticles
Caveospheres
Monoclonal antibodes
MAb
Immunotherapy
Caveolin-1
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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