The use of microwave-assisted solid-phase peptide synthesis and click chemistry for the synthesis of vaccine candidates against hookworm infection

Ahmad Fuaad, Abdullah A. H., Skwarczynski, Mariusz and Toth, Istvan (2016). The use of microwave-assisted solid-phase peptide synthesis and click chemistry for the synthesis of vaccine candidates against hookworm infection. In Thomas, Sunil (Ed.), Vaccine design: methods and protocols: volume 1: vaccines for human diseases (pp. 639-653) New York, NY United States: Springer New York. doi:10.1007/978-1-4939-3387-7_36


Author Ahmad Fuaad, Abdullah A. H.
Skwarczynski, Mariusz
Toth, Istvan
Title of chapter The use of microwave-assisted solid-phase peptide synthesis and click chemistry for the synthesis of vaccine candidates against hookworm infection
Title of book Vaccine design: methods and protocols: volume 1: vaccines for human diseases
Place of Publication New York, NY United States
Publisher Springer New York
Publication Year 2016
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-3387-7_36
Open Access Status Not Open Access
Year available 2016
Series Methods in molecular biology
ISBN 9781493933853
9781493933877
ISSN 1064-3745
Editor Thomas, Sunil
Volume number 1403
Chapter number 36
Start page 639
End page 653
Total pages 15
Total chapters 49
Collection year 2017
Language eng
Abstract/Summary A protein-based vaccine approach against hookworm infection has failed to deliver the expected outcome, due to a problem with an allergic response in the patient or difficulties in the proteins’ production. This implication could be overcome by using a chemically synthesized peptide-based vaccine approach. This approach utilizes minimal pathogenic components that are necessary for the stimulation of the immune response without triggering adverse side effects. To boost the peptide’s immunogenicity, a lipid core peptide (LCP) system can be utilized as a carrier molecule/immunostimulant. This chapter describes in detail the synthesizing of protected lipoamino acid, the self-adjuvanting moiety (LCP core), the peptide epitope, and the final vaccine candidate. The subunit peptide and the LCP core were synthesized using microwave-assisted solid-phase peptide synthesis (SPPS). Then the final hookworm vaccine construct was assembled using the copper-catalyzed azide-alkyne cycloaddition, or “click,” reaction.
Keyword Synthetic vaccine
Peptide-based vaccine
Fmoc-solid-phase peptide synthesis
Microwave-assisted solid-phase peptide synthesis
Copper-catalyzed azide-alkyne cycloaddition
Click chemistry
Hookworm
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

 
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Created: Fri, 06 May 2016, 09:48:51 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences