Measurement of osmotic second virial coefficients by zonal size-exclusion chromatography

Winzor, Donald J. (2016) Measurement of osmotic second virial coefficients by zonal size-exclusion chromatography. Analytical Biochemistry, 504 1: 59-63. doi:10.1016/j.ab.2016.04.003


Author Winzor, Donald J.
Title Measurement of osmotic second virial coefficients by zonal size-exclusion chromatography
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2016-04-16
Sub-type Article (original research)
DOI 10.1016/j.ab.2016.04.003
Open Access Status Not Open Access
Volume 504
Issue 1
Start page 59
End page 63
Total pages 5
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Numerical simulation of protein migration reflecting linear concentration dependence of the partition isotherm has been used to invalidate a published procedure for measuring osmotic second virial coefficients (B22) by zonal exclusion chromatography. Failure of the zonal procedure to emulate its frontal chromatographic counterpart reflects ambiguity about the solute concentration that should be used to replace the applied concentration in the rigorous quantitative expression for frontal migration; the recommended use of the peak concentration in the eluted zone is incorrect on theoretical grounds. Furthermore, the claim for its validation on empirical grounds has been traced to the use of inappropriate B22 magnitudes as the standards against which the experimentally derived values were being tested.
Keyword Bovine serum albumin
Lysozyme
Second virial coefficient
Size-exclusion chromatography
Thermodynamic nonideality
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 06 May 2016, 09:27:53 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences