Development of a novel technique to measure corneal nerve migration rate

Edwards, Katie, Pritchard, Nicola, Poole, Christopher, Dehghani, Cirous, Al Rashah, Khaled, Russell, Anthony, Malik, Rayaz A. and Efron, Nathan (2016) Development of a novel technique to measure corneal nerve migration rate. Cornea, 35 5: 700-705. doi:10.1097/ICO.0000000000000790


Author Edwards, Katie
Pritchard, Nicola
Poole, Christopher
Dehghani, Cirous
Al Rashah, Khaled
Russell, Anthony
Malik, Rayaz A.
Efron, Nathan
Title Development of a novel technique to measure corneal nerve migration rate
Journal name Cornea   Check publisher's open access policy
ISSN 1536-4798
0277-3740
Publication date 2016-05
Year available 2016
Sub-type Article (original research)
DOI 10.1097/ICO.0000000000000790
Open Access Status Not Open Access
Volume 35
Issue 5
Start page 700
End page 705
Total pages 6
Place of publication Philadelphia, PA, United States
Publisher Lippincott Williams & Wilkins
Collection year 2017
Language eng
Formatted abstract
Purpose: We have developed a novel technique to measure in vivo corneal nerve migration.

Methods: Wide-field montages of the subbasal corneal nerve plexus were generated at baseline and after 3 weeks. The 2 montages were manually examined side by side to identify a referent landmark in the inferior whorl region and 20 additional nerve landmarks throughout each montage. A software program was developed to measure nerve migration by quantifying the movement of the nerve landmarks relative to the inferior whorl landmark over the 3-week period. To illustrate the utility of this technique, nerve migration was measured in 2 individuals with diabetes (one with and the other without neuropathy) and a healthy control participant.

Results: The average nerve migration rate was calculated to be 18.4, 49.9, and 41.5 mm/wk for the diabetic individuals with and without neuropathy and the control participant, respectively. The number of landmarks for tracking nerve migration in the participants was 26, 21, and 20, and they were at an average distance of 1500, 1940, and 1461 mm, from the whorl, respectively. The rate of migration depended on the distance from the whorl; hence, linear equations were generated for each subject for comparison.

Conclusions: This novel imaging technique allows rapid measurement of in vivo corneal nerve migration. The results indicate that diabetic neuropathy may be associated with reduced nerve migration; however, because of the high level of manual input required in this technique and the apparent complex characteristics of corneal nerve migration, repeatability and characterization studies are needed.
Keyword Cornea
Diabetes
Nerves
Neuropathy
Regeneration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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