Critical analysis of hydrogen production from mixed culture fermentation under thermophilic condition (60 °C)

Zheng, Hang, Zeng, Raymond J., O'Sullivan, Cathryn and Clarke, William P. (2016) Critical analysis of hydrogen production from mixed culture fermentation under thermophilic condition (60 °C). Applied Microbiology and Biotechnology, 100 11: 5165-5176. doi:10.1007/s00253-016-7482-z


Author Zheng, Hang
Zeng, Raymond J.
O'Sullivan, Cathryn
Clarke, William P.
Title Critical analysis of hydrogen production from mixed culture fermentation under thermophilic condition (60 °C)
Journal name Applied Microbiology and Biotechnology   Check publisher's open access policy
ISSN 1432-0614
0175-7598
Publication date 2016-06
Year available 2016
Sub-type Article (original research)
DOI 10.1007/s00253-016-7482-z
Open Access Status Not Open Access
Volume 100
Issue 11
Start page 5165
End page 5176
Total pages 12
Place of publication Heidelberg, Germany
Publisher Springer
Collection year 2017
Language eng
Formatted abstract
Bio-hydrogen production from mixed culture fermentation (MCF) of glucose was studied by conducting a comprehensive product measurement and detailed mass balance analysis of their contributions to the final H2 yield. The culture used in this study was enriched on glucose at 60 °C through a sequential batch operation consisting of daily glucose feeds, headspace purging and medium replacement every third day in serum bottles for over 2 years. 2-Bromoethanesulfonate (BES) was only required during the first three 3-day cycles to permanently eliminate methanogenic activity. Daily glucose feeds were fully consumed within 24 h, with a persistent H2 yield of 2.7 ± 0.1 mol H2/mol glucose, even when H2 was allowed to accumulate over the 3-day cycle. The measured H2 production exceeded by 14 % the theoretical production of H2 associated with the fermentation products, dominated by acetate and butyrate. Follow-up experiments using acetate with a 13C-labelled methyl group showed that the excess H2 production was not due to acetate oxidation. Chemical formula analysis of the biomass showed a more reduced form of C5H11.8O2.1N1.1 suggesting that the biomass formation may even consume produced H2 from fermentation.
Keyword Bio-hydrogen
Biomass formation
Excess H2 production
Mixed culture fermentation
Stable isotope
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Earth Sciences Publications
School of Civil Engineering Publications
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