Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice

Futrega, Kathryn, Lott, William B. and Doran, Michael R. (2016) Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice. Scientific Reports, 6 . doi:10.1038/srep23886


Author Futrega, Kathryn
Lott, William B.
Doran, Michael R.
Title Direct bone marrow HSC transplantation enhances local engraftment at the expense of systemic engraftment in NSG mice
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-04-11
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep23886
Open Access Status DOI
Volume 6
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Formatted abstract
Direct bone marrow (BM) injection has been proposed as a strategy to bypass homing inefficiencies associated with intravenous (IV) hematopoietic stem cell (HSC) transplantation. Despite physical delivery into the BM cavity, many donor cells are rapidly redistributed by vascular perfusion, perhaps compromising efficacy. Anchoring donor cells to 3-dimensional (3D) multicellular spheroids, formed from mesenchymal stem/stromal cells (MSC) might improve direct BM transplantation. To test this hypothesis, relevant combinations of human umbilical cord blood-derived CD34+ cells and BM-derived MSC were transplanted into NOD/SCID gamma (NSG) mice using either IV or intrafemoral (IF) routes. IF transplantation resulted in higher human CD45+ and CD34+ cell engraftment within injected femurs relative to distal femurs regardless of cell combination, but did not improve overall CD45+ engraftment at 8 weeks. Analysis within individual mice revealed that despite engraftment reaching near saturation within the injected femur, engraftment at distal hematopoietic sites including peripheral blood, spleen and non-injected femur, could be poor. Our data suggest that the retention of human HSC within the BM following direct BM injection enhances local chimerism at the expense of systemic chimerism in this xenogeneic model.
Keyword Hematopoietic stem cells
Mesenchymal stem cells
Preclinical research
Stem-cell research
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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