Osteocyte expression of caspase-3, COX-2, IL-6 and sclerostin are spatially and temporally associated following stress fracture initiation.

Wu, Andy C., Kidd, Lisa J., Cowling, Nicholas R., Kelly, Wendy L. and Forwood, Mark R. (2014) Osteocyte expression of caspase-3, COX-2, IL-6 and sclerostin are spatially and temporally associated following stress fracture initiation.. BoneKEy Reports, 3 571: 1-7. doi:10.1038/bonekey.2014.66


Author Wu, Andy C.
Kidd, Lisa J.
Cowling, Nicholas R.
Kelly, Wendy L.
Forwood, Mark R.
Title Osteocyte expression of caspase-3, COX-2, IL-6 and sclerostin are spatially and temporally associated following stress fracture initiation.
Journal name BoneKEy Reports   Check publisher's open access policy
ISSN 2047-6396
Publication date 2014-09-03
Sub-type Article (original research)
DOI 10.1038/bonekey.2014.66
Open Access Status Not Open Access
Volume 3
Issue 571
Start page 1
End page 7
Total pages 7
Place of publication Washington, DC 20036 United States
Publisher International Bone and Mineral Society
Language eng
Abstract Stress fractures (SFxs) are debilitating injuries and exact mechanisms that initiate their repair incompletely understood. We hypothesised that osteocyte apoptosis and expression of cytokines and proteins such as sclerostin, VEGF, TGF-β, COX-2 and IL-6 were early signalling events to facilitate the formation of periosteal woven bone and recruitment of osteoclast precursors to the site of remodelling. A SFx was created in the right ulna of mature female wistar rats using cyclic end loading. Rats were killed 1, 4 and 7 days after loading (n=5 per group). Standard histological staining was used to examine SFx morphology and immunohistochemistry to detect the localisation of these proteins and in situ hybridisation to detect mRNA along the SFx line or gene expression to quantify the target genes. Unloaded ulnae served as controls. The labelling index of caspase-3, COX-2 and IL-6 was significantly elevated in the region of SFxs at all time points compared with controls (P<0.001). In addition, the labelling index of sclerostin protein was significantly reduced in osteocytes adjacent to the SFx region when compared with controls at all three time points (P<0.001). Both VEGF and TGF-β expressions were only localised in the woven bone. These data reinforce the involvement of osteocyte apoptosis in the healing of fatigue damage in bone, and demonstrate that local regulation of sclerostin, COX-2 and IL-6 are important signalling events associated with new bone formation and SFx remodelling.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
School of Veterinary Science Publications
 
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Created: Tue, 26 Apr 2016, 13:29:32 EST by Andy Wu on behalf of Mater Research Institute-UQ