Behavioural effects of adult Vitamin D deficiency in BALB/c mice are not associated with proliferation or survival of neurons in the adult hippocampus

Groves, Natalie J., Bradford, DanaKai, Sullivan, Robert K. P., Conn, Kyna-Anne, Aljelaify, Rasha Fahad, McGrath, John J. and Burne, Thomas H. J. (2016) Behavioural effects of adult Vitamin D deficiency in BALB/c mice are not associated with proliferation or survival of neurons in the adult hippocampus. PLoS ONE, 11 4: . doi:10.1371/journal.pone.0152328


Author Groves, Natalie J.
Bradford, DanaKai
Sullivan, Robert K. P.
Conn, Kyna-Anne
Aljelaify, Rasha Fahad
McGrath, John J.
Burne, Thomas H. J.
Title Behavioural effects of adult Vitamin D deficiency in BALB/c mice are not associated with proliferation or survival of neurons in the adult hippocampus
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-04-04
Sub-type Article (original research)
DOI 10.1371/journal.pone.0152328
Open Access Status DOI
Volume 11
Issue 4
Total pages 14
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2017
Language eng
Formatted abstract
Epidemiological studies have shown that up to one third of adults have insufficient levels of vitamin D and there is an association between low vitamin D concentrations and adverse brain outcomes, such as depression. Vitamin D has been shown to be involved in processes associated with neurogenesis during development. Therefore, the aim of this study was to test the hypothesis that adult vitamin D (AVD) deficiency in BALB/c mice was associated with (a) adult hippocampal neurogenesis at baseline, b) following 6 weeks of voluntary wheel running and (c) a depressive-like phenotype on the forced swim test (FST), which may be linked to alterations in hippocampal neurogenesis. We assessed proliferation and survival of adult born hippocampal neurons by counting the number of cells positive for Ki67 and doublecortin (DCX), and incorporation of 5-Bromo-2’-Deoxyuridine (BrdU) within newly born mature neurons using immunohistochemistry. There were no significant effects of diet on number of Ki67+, DCX+ or BrdU+ cells in the dentate gyrus. All mice showed significantly increased number of Ki67+ cells and BrdU incorporation, and decreased immobility time in the FST, after voluntary wheel running. A significant correlation was found in control mice between immobility time in the FST and level of hippocampal neurogenesis, however, no such correlation was found for AVD-deficient mice. We conclude that AVD deficiency was not associated with impaired proliferation or survival of adult born neurons in BALB/c mice and that the impact on rodent behaviour may not be due to altered neurogenesis per se, but to altered function of new hippocampal neurons or processes independent of adult neurogenesis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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