Patterns of differentially expressed genes in oral mucosal lesions visualised under autofluorescence (VELscope™)

Kordbacheh, F., Bhatia, N. and Farah, C. S. (2016) Patterns of differentially expressed genes in oral mucosal lesions visualised under autofluorescence (VELscope™). Oral Diseases, 22 4: 285-296. doi:10.1111/odi.12438


Author Kordbacheh, F.
Bhatia, N.
Farah, C. S.
Title Patterns of differentially expressed genes in oral mucosal lesions visualised under autofluorescence (VELscope™)
Journal name Oral Diseases   Check publisher's open access policy
ISSN 1601-0825
1354-523X
Publication date 2016-05-01
Year available 2016
Sub-type Article (original research)
DOI 10.1111/odi.12438
Open Access Status Not yet assessed
Volume 22
Issue 4
Start page 285
End page 296
Total pages 12
Place of publication Hoboken, NJ, United States
Publisher Wiley-Blackwell Publishing
Collection year 2017
Language eng
Formatted abstract
Objectives: We aimed to elucidate the molecular pathways associated with fluorescence properties of oral potentially malignant disorders (OPMD) visualised under direct tissue autofluorescence (VELscope™).

Materials and Methods: Forty-two oral mucosal biopsies correlated with clinical fluorescence characteristics were categorised based on histopathological diagnosis. Four oral squamous cell carcinoma (OSCC), 15 oral epithelial dysplasia (OED), nine oral lichen planus (OLP) and 14 oral epithelial hyperplasia (OEH) presenting with three fluorescence patterns including retained fluorescence (RF), loss of fluorescence (LAF) with blanching (LB) and LAF with no blanching (LNB) were assessed. Relative gene expression was measured through RNA sequencing.

Results: Although each lesion type had a specific set of histology-related differentially expressed genes (DEGs), all tested samples shared a number of DEGs, and we could not identify a discriminatory component between histological groups. Gene ontology enrichment revealed LAF in OEH was mostly due to changes in inflammation, cell cycle regulation and apoptosis, while in OED was due to inflammation, angiogenesis and extracellular matrix remodelling. Inflammatory reactions were associated with diascopic fluorescence (DF) for both OEH and OED.

Conclusion: Uncovering the molecular mechanisms underlying LAF and DF may lead to reduction in the number of false-positive and false-negative findings and improve the efficacy and utility of VELscope™.
Keyword Cellular pathways/molecular mechanisms
Diascopic fluorescence
Loss of tissue autofluorescence
Oral cancer
Oral potentially malignant disorders
RNA sequencing
VELscope™
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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