Minimizing matrix effects for the accurate quantification of 25-hydroxyvitamin D metabolites in dried blood spots by LC-MS/MS

Kvaskoff, David, Heath, Alicia K., Simila, Henry A., Ko, Pauline, English, Dallas R. and Eyles, Darryl W. (2016) Minimizing matrix effects for the accurate quantification of 25-hydroxyvitamin D metabolites in dried blood spots by LC-MS/MS. Clinical Chemistry, 62 4: 639-646. doi:10.1373/clinchem.2015.251538


Author Kvaskoff, David
Heath, Alicia K.
Simila, Henry A.
Ko, Pauline
English, Dallas R.
Eyles, Darryl W.
Title Minimizing matrix effects for the accurate quantification of 25-hydroxyvitamin D metabolites in dried blood spots by LC-MS/MS
Journal name Clinical Chemistry   Check publisher's open access policy
ISSN 1530-8561
0009-9147
Publication date 2016-04-01
Year available 2016
Sub-type Article (original research)
DOI 10.1373/clinchem.2015.251538
Open Access Status Not Open Access
Volume 62
Issue 4
Start page 639
End page 646
Total pages 8
Place of publication Washington, DC, United States
Publisher American Association for Clinical Chemistry
Collection year 2017
Language eng
Formatted abstract
Background: The noncalcemic actions of Vitamin D in multiple organs are now widely recognized. Vitamin D status has been linked with a wide variety of conditions, which has led to an increasing demand for Vitamin D screening. In particular, there is intense interest in the impact of Vitamin D on a variety of developmental conditions. The most readily accessible pediatric samples are dried blood spots, and health organizations are increasingly archiving such samples for later assessment of the antecedents of disease.

Methods: In 2009, we developed a method to quantify the major circulatory form of Vitamin D, 25-hydroxyVitamin D, in archived dried blood spots. Over the last 6 years, we have made substantial alterations to the published method to enhance throughput, sensitivity, and assay robustness.

Results: With the alterations, the assay was 3 times faster than the previously published assay and had a > 10-fold increase in signal strength. Intraassay imprecision decreased from 13.4% to 6.9%, and there was a 5-fold reduction in interfering phospholipids. In actual use over 2 years, the assay showed an interassay imprecision of 11.6%.

Conclusions: This assay has performed reliably over the past 6 years. The practical changes we have made should allow clinical chemists to successfully adapt this method.
Keyword LC-MS/MS
Matrix effects
Vitamin D
25-hydroxyvitamin D
25OHD
Dried blood spots
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
 
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