The development and characterisation of a SV40 T-antigen expressing cell line of human hepatic origin

Harvey, T. J., Macnaughton, T. B. and Gowans, E. J. (1997) The development and characterisation of a SV40 T-antigen expressing cell line of human hepatic origin. Journal of Virological Methods, 65 1: 67-74. doi:10.1016/S0166-0934(96)02170-2


Author Harvey, T. J.
Macnaughton, T. B.
Gowans, E. J.
Title The development and characterisation of a SV40 T-antigen expressing cell line of human hepatic origin
Journal name Journal of Virological Methods   Check publisher's open access policy
ISSN 1879-0984
0166-0934
Publication date 1997-04
Sub-type Article (original research)
DOI 10.1016/S0166-0934(96)02170-2
Open Access Status Not Open Access
Volume 65
Issue 1
Start page 67
End page 74
Total pages 8
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract COS7 cells and other cell lines expressing the SV40 large T-antigen, have been of great benefit in transfection studies using transient expression vectors which contain the SV40 origin of replication, as these cells allow plasmid replication to a high copy number. As no T-antigen expressing cell line derived from a well characterised hepatocyte-like continuous cell line currently exists, the establishment of such a cell line for studies which require expression of hepatocyte-specific factors would be extremely useful. A HepG2-derived stable cell line (THT1) was therefore developed which demonstrates a high level of transfection efficiency whilst retaining hepatocyte-like features, such as the production of hepatitis B virus. The THT1 cell line displayed chromosomal integration of the SV40 T-antigen gene and nuclear expression of the antigen. The cell line also maintained the general morphological features of its parent cell line, and showed an increased rate of cell growth.
Keyword Hepatitis B Virus
Stable cell line
SV40 large T-antigen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Biomedical Sciences Publications
 
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Created: Wed, 20 Apr 2016, 16:16:15 EST by Elisha Bignell on behalf of School of Biomedical Sciences