A broad-standard technique for correcting for band broadening in size-exclusion chromatography

Zhang, Peng, Mazoyer, Paul and Gilbert, Robert G. (2016) A broad-standard technique for correcting for band broadening in size-exclusion chromatography. Journal of Chromatography A, 1443 267-271. doi:10.1016/j.chroma.2016.03.030

Author Zhang, Peng
Mazoyer, Paul
Gilbert, Robert G.
Title A broad-standard technique for correcting for band broadening in size-exclusion chromatography
Journal name Journal of Chromatography A   Check publisher's open access policy
ISSN 1873-3778
Publication date 2016-04-22
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.chroma.2016.03.030
Open Access Status Not Open Access
Volume 1443
Start page 267
End page 271
Total pages 5
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Collection year 2017
Language eng
Formatted abstract
Band broadening in size-exclusion chromatography (SEC) is always present to some extent. Broadening effects on averages such as the weight- and number average molecular weights (MW and Mn respectively) are minimal with modern SEC systems. However, broadening distorts the shape of the true molecular weight distribution (MWD), which causes problems if one wants to compare the detailed form of the MWD to a model. An addition to current methods for overcoming this problem is presented. One starts with a sufficiently wide range of samples whose exact values of Mn and. MW have been measured by non-SEC methods (e.g. by fluorimetry and light scattering, respectively, of the sample without size separation). A true (unbroadened) molecular weight distribution for a sample can be obtained by deconvolution (here using a maximum-entropy algorithm) by fitting SEC data for these samples to these exact Mn and MW values to find the values of the parameters in a sufficiently flexible assumed broadening function. This was modelled using simulated band broadening and subsequent deconvolution, with the broadening parameters least-squares fitted to the "exact" sets of values of Mn and MWThe results show that if these Mn and MW values are for a series of broad (not narrow) standards covering a sufficient range of molecular weight, then after deconvolution, a good representation of the original molecular weight distribution used in the simulation is obtained. The method should prove useful for water-soluble polymers, for which it is often difficult to obtain narrow standards of a wide range of molecular weight, as required in a number of well-established methods for correcting for band broadening.
Keyword Band broadening
Size exclusion chromatography (SEC)
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
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