Differential response of patient-derived primary glioblastoma cells to environmental stiffness

Grundy, Thomas James, De Leon, Ellen, Griffin, Kaitlyn Rose, Stringer, Brett William, Day, Bryan William, Fabry, Ben, Cooper-White, Justin and O'Neill, Geraldine Margaret (2016) Differential response of patient-derived primary glioblastoma cells to environmental stiffness. Scientific Reports, 6 23353: 1-10. doi:10.1038/srep23353

Author Grundy, Thomas James
De Leon, Ellen
Griffin, Kaitlyn Rose
Stringer, Brett William
Day, Bryan William
Fabry, Ben
Cooper-White, Justin
O'Neill, Geraldine Margaret
Title Differential response of patient-derived primary glioblastoma cells to environmental stiffness
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-03-21
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep23353
Open Access Status DOI
Volume 6
Issue 23353
Start page 1
End page 10
Total pages 10
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Abstract The ability of cancer cells to sense external mechanical forces has emerged as a significant factor in the promotion of cancer invasion. Currently there are conflicting reports in the literature with regard to whether glioblastoma (GBM) brain cancer cell migration and invasion is rigidity-sensitive. In order to address this question we have compared the rigidity-response of primary patient-derived GBM lines. Cells were plated on polyacrylamide gels of defined rigidity that reflect the diversity of the brain tissue mechanical environment, and cell morphology and migration were analysed by time-lapse microscopy. Invasiveness was assessed in multicellular spheroids embedded in 3D matrigel cultures. Our data reveal a range of rigidity-dependent responses between the patient-derived cell lines, from reduced migration on the most compliant tissue stiffness to those that are insensitive to substrate rigidity and are equally migratory irrespective of the underlying substrate stiffness. Notably, the rigidity-insensitive GBM cells show the greatest invasive capacity in soft 3D matrigel cultures. Collectively our data confirm both rigidity-dependent and independent behaviour in primary GBM patient-derived cells.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
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