Non-infectious virus-like particles for the validation of membrane integrity and column performance in bioprocessing

Wang, Hui and Middelberg, Anton P. J. (2016) Non-infectious virus-like particles for the validation of membrane integrity and column performance in bioprocessing. Food and Bioproducts Processing, 98 327-332. doi:10.1016/j.fbp.2016.03.001


Author Wang, Hui
Middelberg, Anton P. J.
Title Non-infectious virus-like particles for the validation of membrane integrity and column performance in bioprocessing
Journal name Food and Bioproducts Processing   Check publisher's open access policy
ISSN 0960-3085
1744-3571
Publication date 2016-04-01
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.fbp.2016.03.001
Open Access Status Not Open Access
Volume 98
Start page 327
End page 332
Total pages 6
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Effective viral clearance is critical in both water treatment and biopharmaceutical manufacture to protect public health. In this study, virus-like particle (VLP) was used as a surrogate for membrane and column performance assessments. A reliable ELISA method was developed to quantify VLPs with high sensitivity and specificity. Log10 reduction value (LRV) was measured in a scaled-down manner by mimicking the unit operation using VLPs. This study provides a potential to utilise VLPs in the real-time validation on membrane and column performance in water-treatment and biopharmaceutical manufacturing industries.
Keyword Virus-like particle
Virus removal
Membrane filtration
Anion exchange chromatography
Water treatment
Biopharmaceutical manufacture
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
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