A three-dimensional scaffold-based system for modeling the bone marrow tissue

Gheisari, Yousof, Vasei, Mohammad, Shafiee, Abbas, Soleimani, Masoud, Seyedjafari, Ehsan, Omidhkoda, Azadeh, Langroudi, Ladan and Ahmadbeigi, Naser (2016) A three-dimensional scaffold-based system for modeling the bone marrow tissue. Stem Cells and Development, 25 6: 492-498. doi:10.1089/scd.2015.0182


Author Gheisari, Yousof
Vasei, Mohammad
Shafiee, Abbas
Soleimani, Masoud
Seyedjafari, Ehsan
Omidhkoda, Azadeh
Langroudi, Ladan
Ahmadbeigi, Naser
Title A three-dimensional scaffold-based system for modeling the bone marrow tissue
Journal name Stem Cells and Development   Check publisher's open access policy
ISSN 1547-3287
1557-8534
Publication date 2016-03-15
Year available 2016
Sub-type Article (original research)
DOI 10.1089/scd.2015.0182
Open Access Status Not Open Access
Volume 25
Issue 6
Start page 492
End page 498
Total pages 7
Place of publication New Rochelle, NY United States
Publisher Mary Ann Liebert, Inc. Publishers
Collection year 2017
Language eng
Abstract Hematopoietic stem and progenitor cells (HPC) niche, consisting of HPC and their surrounding stromal components, is the fundamental unit for bone marrow (BM) tissue engineering. Previously, mouse BM-derived cell complexes with HPC niche unit properties called “niche-like units” were isolated and characterized. This study was aimed to evaluate the possibility of bioengineering marrow tissue in heterotypic sites using niche-like units in combination with three-dimensional scaffolds. BM niche-like units were isolated from GFP-transgenic C57BL/6 mice and seeded on electrospun poly (L-lactide) nanofiber scaffolds, which were then roll-folded and aseptically implanted into the peritoneal cavity of irradiated wild-type mice. One month after implantation, donor-derived cells were detected in peripheral blood of the recipients and contributed to restoration of all blood lineages. The transplanted bioengineered tissue histologically resembled native BM structure and was connected to the mouse systemic circulation. Long-term self-renewal was confirmed by serial transplantation into tertiary recipients. In conclusion, this study establishes a novel system for BM tissue engineering, which can be used to improve the HPC transplantation outcomes especially in cases where HPC niche is damaged and also as an in vivo model to test the effects of different factors on hematopoiesis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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