Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans

Zacchi, Lucia F., Selmecki, Anna M., Berman, Judith and Davis, Dana A. (2010) Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans. PLoS One, 5 5: e10629.1-e10629.12. doi:10.1371/journal.pone.0010629


Author Zacchi, Lucia F.
Selmecki, Anna M.
Berman, Judith
Davis, Dana A.
Title Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans
Formatted title
Low dosage of histone H4 leads to growth defects and morphological changes in Candida albicans
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2010
Sub-type Article (original research)
DOI 10.1371/journal.pone.0010629
Open Access Status DOI
Volume 5
Issue 5
Start page e10629.1
End page e10629.12
Total pages 12
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Language eng
Abstract Chromatin function depends on adequate histone stoichiometry. Alterations in histone dosage affect transcription and chromosome segregation, leading to growth defects and aneuploidies. In the fungal pathogen Candida albicans, aneuploidy formation is associated with antifungal resistance and pathogenesis. Histone modifying enzymes and chromatin remodeling proteins are also required for pathogenesis. However, little is known about the mechanisms that generate aneuploidies or about the epigenetic mechanisms that shape the response of C. albicans to the host environment. Here, we determined the impact of histone H4 deficit in the growth and colony morphology of C. albicans. We found that C. albicans requires at least two of the four alleles that code for histone H4 (HHF1 and HHF22) to grow normally. Strains with only one histone H4 allele show a severe growth defect and unstable colony morphology, and produce faster-growing, morphologically stable suppressors. Segmental or whole chromosomal trisomies that increased wild-type histone H4 copy number were the preferred mechanism of suppression. This is the first study of a core nucleosomal histone in C. albicans, and constitutes the prelude to future, more detailed research on the function of histone H4 in this important fungal pathogen.
Keyword Candida albicans
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Thu, 07 Apr 2016, 21:22:23 EST by Lucia Zacchi on behalf of School of Chemistry & Molecular Biosciences