Mds3 regulates morphogenesis in Candida albicans through the TOR pathway

Zacchi, Lucia F., Gomez-Raja, Jonathan and Davis, Dana A. (2010) Mds3 regulates morphogenesis in Candida albicans through the TOR pathway. Molecular and Cellular Biology, 30 14: 3695-3710. doi:10.1128/MCB.01540-09

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Author Zacchi, Lucia F.
Gomez-Raja, Jonathan
Davis, Dana A.
Title Mds3 regulates morphogenesis in Candida albicans through the TOR pathway
Formatted title
Mds3 regulates morphogenesis in Candida albicans through the TOR pathway
Journal name Molecular and Cellular Biology   Check publisher's open access policy
ISSN 0270-7306
1098-5549
Publication date 2010
Sub-type Article (original research)
DOI 10.1128/MCB.01540-09
Open Access Status File (Publisher version)
Volume 30
Issue 14
Start page 3695
End page 3710
Total pages 16
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
The success of Candida albicans as a major human fungal pathogen is dependent on its ability to colonize and survive as a commensal on diverse mucosal surfaces. One trait required for survival and virulence in the host is the morphogenetic yeast-to-hypha transition. Mds3 was identified as a regulator of pH-dependent morphogenesis that functions in parallel with the classic Rim101 pH-sensing pathway. Microarray analyses revealed that mds3Δ/Δ cells had an expression profile indicative of a hyperactive TOR pathway, including the preferential expression of genes encoding ribosomal proteins and a decreased expression of genes involved in nitrogen source utilization. The transcriptional and morphological defects of the mds3Δ/Δ mutant were rescued by rapamycin, an inhibitor of TOR, and this rescue was lost in strains carrying the rapamycin-resistant TOR1-1 allele or an rbp1Δ/Δ deletion. Rapamycin also rescued the transcriptional and morphological defects associated with the loss of Sit4, a TOR pathway effector, but not the loss of Rim101 or Ras1. The sit4Δ/Δ and mds3Δ/Δ mutants had additional phenotypic similarities, suggesting that Sit4 and Mds3 function similarly in the TOR pathway. Finally, we found that Mds3 and Sit4 coimmunoprecipitate. Thus, Mds3 is a new member of the TOR pathway that contributes to morphogenesis in C. albicans as a regulator of this key morphogenetic pathway.
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Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Chemistry and Molecular Biosciences
 
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Created: Thu, 07 Apr 2016, 21:20:38 EST by Lucia Zacchi on behalf of School of Chemistry & Molecular Biosciences