Quantitation of folates and their catabolites in blood plasma, erythrocytes, and urine by stable isotope dilution assays

Moench, Sabine, Netzel, Michael, Netzel, Gabriele and Rychlik, Michael (2010) Quantitation of folates and their catabolites in blood plasma, erythrocytes, and urine by stable isotope dilution assays. Analytical Biochemistry, 398 2: 150-160. doi:10.1016/j.ab.2009.11.007


Author Moench, Sabine
Netzel, Michael
Netzel, Gabriele
Rychlik, Michael
Title Quantitation of folates and their catabolites in blood plasma, erythrocytes, and urine by stable isotope dilution assays
Journal name Analytical Biochemistry   Check publisher's open access policy
ISSN 0003-2697
1096-0309
Publication date 2010-03-15
Year available 2010
Sub-type Article (original research)
DOI 10.1016/j.ab.2009.11.007
Open Access Status Not Open Access
Volume 398
Issue 2
Start page 150
End page 160
Total pages 11
Place of publication Philadelphia, PA, United States
Publisher Elsevier
Language eng
Formatted abstract
New stable isotope dilution assays were developed for the simultaneous quantitation of the folates 5-methyltetrahydrofolic acid, 5-formyltetrahydrofolic acid, tetrahydrofolic acid, 10-formylfolic acid, and folic acid as well as for their catabolites para-aminobenzoylglutamate (pABG) and acetyl-para-aminobenzoylglutamate (ApABG) in clinical samples. The methods were based on cleanup by strong anion exchange followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection. Deuterated analogues of the folates and [13C5]-labeled isotopologues of the catabolites were applied as the internal standards in stable isotope dilution assays. Extraction in 4-morpholineethanesulfonic acid (MES) buffer at pH 5.0 ensured the optimum stability of folates and, in combination with solid-phase extraction (SPE) based on strong anion exchange, resulted in higher recoveries compared with other combinations of extraction buffers and SPE. The method was sensitive enough to detect pABG in plasma generally and unmetabolized folic acid in the plasma of a volunteer after oral dosage of an aqueous folic acid solution. The sum of folate catabolites increased by a factor of 2 in the urine of the latter volunteer, compared with that resulting when only water was dosed.
Keyword Folate catabolites
Folates
LC-MS/MS
Plasma
Red blood cells
Stable isotope dilution assay
Urine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Queensland Alliance for Agriculture and Food Innovation
 
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