Fibrin-fiber architecture influences cell spreading and differentiation

Bruekers, Stephanie M. C., Jaspers, Maarten, Hendriks, Jose M. A., Kurniawan, Nicholas A., Koenderink, Gijsje E., Kouwer, Paul H. J., Rowan, Alan E. and Huck, Wilhelm T. S. (2016) Fibrin-fiber architecture influences cell spreading and differentiation. Cell Adhesion and Migration, 10 4: 1-10. doi:10.1080/19336918.2016.1151607


Author Bruekers, Stephanie M. C.
Jaspers, Maarten
Hendriks, Jose M. A.
Kurniawan, Nicholas A.
Koenderink, Gijsje E.
Kouwer, Paul H. J.
Rowan, Alan E.
Huck, Wilhelm T. S.
Title Fibrin-fiber architecture influences cell spreading and differentiation
Journal name Cell Adhesion and Migration   Check publisher's open access policy
ISSN 1933-6926
1933-6918
Publication date 2016-02-24
Year available 2016
Sub-type Article (original research)
DOI 10.1080/19336918.2016.1151607
Open Access Status Not Open Access
Volume 10
Issue 4
Start page 1
End page 10
Total pages 10
Place of publication Philadelphia, PA, United States
Publisher Taylor & Francis
Collection year 2017
Language eng
Abstract The mechanical and structural properties of the extracellular matrix (ECM) play an important role in regulating cell fate. The natural ECM has a complex fibrillar structure and shows nonlinear mechanical properties, which are both difficult to mimic synthetically. Therefore, systematically testing the influence of ECM properties on cellular behavior is very challenging. In this work we show two different approaches to tune the fibrillar structure and mechanical properties of fibrin hydrogels. Addition of extra thrombin before gelation increases the protein density within the fibrin fibers without significantly altering the mechanical properties of the resulting hydrogel. On the other hand, by forming a composite hydrogel with a synthetic biomimetic polyisocyanide network the protein density within the fibrin fibers decreases, and the mechanics of the composite material can be tuned by the PIC/fibrin mass ratio. The effect of the changes in gel structure and mechanics on cellular behavior are investigated, by studying human mesenchymal stem cell (hMSC) spreading and differentiation on these gels. We find that the trends observed in cell spreading and differentiation cannot be explained by the bulk mechanics of the gels, but correlate to the density of the fibrin fibers the gels are composed of. These findings strongly suggest that the microscopic properties of individual fibers in fibrous networks play an essential role in determining cell behavior.
Keyword Differentiation
Fiber architecture
Fibrin
Hydrogels
Mechanotransduction
Mesenchymal stem cells
Rheology
Strain stiffening
Turbidity
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
 
Versions
Version Filter Type
Citation counts: Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 05 Apr 2016, 00:38:45 EST by System User on behalf of Learning and Research Services (UQ Library)