The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica

Sogabe, Shunsuke, Nakanishi, Nagayasu and Degnan, Bernard M. (2016) The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica. Evodevo, 7 6: 1-13. doi:10.1186/s13227-016-0042-x


Author Sogabe, Shunsuke
Nakanishi, Nagayasu
Degnan, Bernard M.
Title The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Formatted title
The ontogeny of choanocyte chambers during metamorphosis in the demosponge Amphimedon queenslandica
Journal name Evodevo   Check publisher's open access policy
ISSN 2041-9139
Publication date 2016-03
Year available 2016
Sub-type Article (original research)
DOI 10.1186/s13227-016-0042-x
Open Access Status DOI
Volume 7
Issue 6
Start page 1
End page 13
Total pages 13
Place of publication London, United Kingdom
Publisher BioMed Central
Collection year 2017
Language eng
Formatted abstract
Background
The aquiferous body plan of poriferans revolves around internal chambers comprised of choanocytes, a cell type structurally similar to choanoflagellates. These choanocyte chambers perform a range of physiological and developmental functions, including the capture of food and the generation of stem cells. Despite the increasing interest for choanocytes as sponge stem cells, there is limited knowledge on the development of choanocyte chambers. Using a combination of cell lineage tracing, antibody staining and EdU labeling, here we examine the development of choanocytes and the chambers they comprise during metamorphosis in the marine demosponge Amphimedon queenslandica.

Results
Lineage-tracing experiments show that larval epithelial cells transform into mesenchymal pluripotent stem cells, resembling archeocytes, within 24 h of initiating metamorphosis. By 36 h, some of these labeled archeocyte-like cells have differentiated into choanocytes that will form the first postlarval choanocyte chambers. Non-labeled cells also contribute to these primary choanocyte chambers, consistent with these chambers being a chimera of multiple transdifferentiated larval cell types and not the proliferation of a single choanocyte precursor. Moreover, cell proliferation assays demonstrate that, following the initial formation of choanocyte chambers, chambers grow at least partially by the proliferation of choanocytes within the chamber, although recruitment of individual cells into established chambers also appears to occur. EdU labeling of postlarvae and juveniles reveals that choanocyte chambers are the primary location of cell proliferation during metamorphosis.

Conclusion
Our results show that multiple larval cell lineages typically contribute to formation of individual choanocyte chambers at metamorphosis, contrary to previous reports in other species that show sponge choanocyte chambers form clonally. Choanocytes in postlarval and juvenile A. queenslandica chambers can also divide, with choanocyte chambers being the primary location of cell proliferation. Interestingly, the level of cell proliferation varies greatly between chambers and appears to be contingent on the size, location and developmental state of the chamber. Small chambers on the periphery of the body tend to possess more dividing cells. As choanocytes can also dedifferentiate into archeocyte-like cells, cell proliferation in chambers may not only contribute to chamber growth and self-renewal but also increase the number of pluripotent archeocytes.
Keyword Porifera
Choanocyte
Choanocyte chamber
Development
Demospongiae
Archeocyte
Invertebrates
Evolution
Stem cells
Ontogeny
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Google Scholar Search Google Scholar
Created: Sun, 03 Apr 2016, 00:25:03 EST by System User on behalf of School of Biological Sciences