Molecular structure of human-liver glycogen

Deng, Bin, Sullivan, Mitchell A., Chen, Cheng, Li, Jialun, Powell, Prudence O., Hu, Zhenxia and Gilbert, Robert G. (2016) Molecular structure of human-liver glycogen. Plos One, 11 3: . doi:10.1371/journal.pone.0150540


Author Deng, Bin
Sullivan, Mitchell A.
Chen, Cheng
Li, Jialun
Powell, Prudence O.
Hu, Zhenxia
Gilbert, Robert G.
Title Molecular structure of human-liver glycogen
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-03-02
Year available 2016
Sub-type Article (original research)
DOI 10.1371/journal.pone.0150540
Open Access Status DOI
Volume 11
Issue 3
Total pages 10
Place of publication San Francisco, CA, United States
Publisher Public Library of Science
Collection year 2017
Language eng
Formatted abstract
Glycogen is a highly branched glucose polymer which is involved in maintaining bloodsugar homeostasis. Liver glycogen contains large composite α particles made up of linked β particles. Previous studies have shown that the binding which links β particles into α particles is impaired in diabetic mice. The present study reports the first molecular structural characterization of human-liver glycogen from non-diabetic patients, using transmission electron microscopy for morphology and size-exclusion chromatography for the molecular size distribution; the latter is also studied as a function of time during acid hydrolysis in vitro, which is sensitive to certain structural features, particularly glycosidic vs. proteinaceous linkages. The results are compared with those seen in mice and pigs. The molecular structural change during acid hydrolysis is similar in each case, and indicates that the linkage of β into α particles is not glycosidic. This result, and the similar morphology in each case, together imply that human liver glycogen has similar molecular structure to those of mice and pigs. This knowledge will be useful for future diabetes drug targets.
Keyword Glycogens
Molecular structure
Acid hydrolysis
Diabetes drug targets
Liver glycogen
Diabetes
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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