The E3 ubiquitin ligase RNF144B is LPS-inducible in human but not mouse macrophages, and promotes inducible IL-1β expression

Arrifin, Juliana K, Kapetanovic, Ronan, Schaale, Kolja, Gatica-Andrades, Marcela, Blumenthal, Antje, Schroder, Kate and Sweet, Matthew J. (2016) The E3 ubiquitin ligase RNF144B is LPS-inducible in human but not mouse macrophages, and promotes inducible IL-1β expression. Journal of Leukocyte Biology, 100 1: 155-161. doi:10.1189/jlb.2AB0815-339R


Author Arrifin, Juliana K
Kapetanovic, Ronan
Schaale, Kolja
Gatica-Andrades, Marcela
Blumenthal, Antje
Schroder, Kate
Sweet, Matthew J.
Title The E3 ubiquitin ligase RNF144B is LPS-inducible in human but not mouse macrophages, and promotes inducible IL-1β expression
Journal name Journal of Leukocyte Biology   Check publisher's open access policy
ISSN 0741-5400
1938-3673
Publication date 2016
Sub-type Article (original research)
DOI 10.1189/jlb.2AB0815-339R
Volume 100
Issue 1
Start page 155
End page 161
Total pages 7
Place of publication Bethesda, United States
Publisher Federation of American Societies for Experimental Biology
Collection year 2017
Language eng
Formatted abstract
Differences in human and mouse immune responses may partly reflect species-specific adaptations and can provide important insights into human immunity. In this study, we show that RNF144B, which encodes an E3 ubiquitin ligase, was lipopolysaccharide-inducible in primary human macrophages and in human macrophage–like THP-1 cells. In contrast, Rnf144b was not lipopolysaccharide-inducible in several mouse cell populations, including primary macrophages from C57BL/6 and BALB/c mice and RAW264.7 macrophages. Similarly, Rnf144b was not up-regulated by infection of C57BL/6 mice with Escherichia coli. Although the human and mouse RNF144B genes have conserved transcription start sites, cap analysis of gene expression data confirmed that the RNF144B promoter directs transcription in human but not mouse macrophages. The human and mouse RNF144B genes are controlled by highly conserved TATA-containing promoters, but subtle differences in transcription factor binding sites may account for differential regulation. Using gene silencing, we showed that RNF144B is necessary for priming of inflammasome responses in primary human macrophages. Specifically, RNF144B promotes lipopolysaccharide-inducible IL-1b mRNA expression but does not regulate expression of several other lipopolysaccharide-inducible cytokines (e.g., interleukin-10, interferon-γ) or affect expression of inflammasome components or substrates (e.g., procaspase-1, pro-interleukin-18). Our findings thus revealed a species-specific regulatory mechanism for selective inflammasome priming in human macrophages.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
UQ Diamantina Institute Publications
 
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Created: Fri, 01 Apr 2016, 16:00:32 EST by Dr Kate Schroder on behalf of School of Chemistry & Molecular Biosciences