A preliminary study on the effect of wounding on transforming growth factor-beta 1 and cartilage oligomeric matrix protein concentrations in the skin of horses

Dart, Andrew J., Dart, Christina M., Dudhia, Jay, Perkins, Nigel, Canfield, Paul and Smith, Roger K. W. (2011) A preliminary study on the effect of wounding on transforming growth factor-beta 1 and cartilage oligomeric matrix protein concentrations in the skin of horses. Veterinary Surgery, 40 1: 59-65. doi:10.1111/j.1532-950X.2010.00756.x


Author Dart, Andrew J.
Dart, Christina M.
Dudhia, Jay
Perkins, Nigel
Canfield, Paul
Smith, Roger K. W.
Title A preliminary study on the effect of wounding on transforming growth factor-beta 1 and cartilage oligomeric matrix protein concentrations in the skin of horses
Journal name Veterinary Surgery   Check publisher's open access policy
ISSN 0161-3499
1532-950X
Publication date 2011-01-01
Sub-type Article (original research)
DOI 10.1111/j.1532-950X.2010.00756.x
Open Access Status Not Open Access
Volume 40
Issue 1
Start page 59
End page 65
Total pages 7
Place of publication Hoboken, NJ United States
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective: To evaluate whether wound type or site influence the production of cartilage oligomeric matrix protein (COMP) and transforming growth factor β1 (TGF-β1), and determine if there is a correlation between TGF-β1and COMP during healing.

Study Design: Experimental.

Animals: Standardbred horses (n=6), 4–8 years old.

Methods: Six, standardized, full-thickness skin wounds (open, straight, and elliptical) were surgically created on the neck (n=3) and metacarpus (3) on each horse. Wounds were randomly allocated to site and side. Tissue samples were collected before creating wounds and on days 7, 14, and 42. COMP concentration (μg/g dry weight of tissue) was determined using a standard competitive ELISA and TGF-β1 (ng/g dry weight of tissue) was determined using a commercially available sandwich ELISA.

Results: COMP concentrations were higher in intact skin on the neck compared with the metacarpus (P=.02). There was no difference in COMP and TGF-β1 concentrations between the different wound types or sites during healing. There was no correlation between TGF-β1 and COMP during healing.

Conclusions: Within the limitations of the study design, production of COMP during healing of skin wounds does not appear to be influenced by wound type or anatomic site, nor does it appear to be correlated with TGF-β1 concentrations.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 30 Mar 2016, 18:17:25 EST by Nigel Perkins on behalf of School of Veterinary Science