Effects of collecting serial tracheal aspirate and bronchoalveolar lavage samples on the cytological findings of subsequent samples in healthy Standardbred horses

Tee, SY, Dart, AJ, MacDonald, MH, Perkins, NR, Horadogoda, N and Jeffcott, LB (2012) Effects of collecting serial tracheal aspirate and bronchoalveolar lavage samples on the cytological findings of subsequent samples in healthy Standardbred horses. Australian Veterinary Journal, 90 7: 247-251. doi:10.1111/j.1751-0813.2012.00950.x


Author Tee, SY
Dart, AJ
MacDonald, MH
Perkins, NR
Horadogoda, N
Jeffcott, LB
Title Effects of collecting serial tracheal aspirate and bronchoalveolar lavage samples on the cytological findings of subsequent samples in healthy Standardbred horses
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 2012-07-01
Year available 2012
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.2012.00950.x
Open Access Status Not Open Access
Volume 90
Issue 7
Start page 247
End page 251
Total pages 5
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective To evaluate the effect of collecting serial tracheal aspirate (TA) and bronchoalveolar lavage (BAL) samples on the cytological findings of subsequent fluid samples obtained from horses without clinical signs of respiratory disease.

Study design Experimental.

Study population Six healthy Standardbred horses.

Methods Endoscopically-guided TA samples, and BAL samples collected using the blind field technique were obtained from the six horses on days 1, 2, 3, 4, 5, 12, and 17. On day 17, horses were sampled three times: at baseline and at 2.5 h and 4 h apart. The differential cytology of the fluid samples collected at each time point was expressed as percentages and compared statistically.

Results There was a significant increase in neutrophil percentage in the TA samples taken at day 17 (at 2.5 h but not at 4 h apart). There was no significant change in the neutrophil percentages in the TA samples when repeated samples were taken ≥24 h apart. There was no significant change in the neutrophil percentages in the BAL fluid at any collection point. There were inconsistent changes in the percentages of lymphocytes and macrophages in the BAL fluid over time, but these remained within normal reference ranges and were considered clinically insignificant.

Conclusions Serial TA and BAL samples can be taken at 24 h intervals without affecting the cytological findings of subsequent fluid samples collected using the techniques described.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 30 Mar 2016, 18:00:50 EST by Nigel Perkins on behalf of School of Veterinary Science