Effects of intra-articular sodium pentosan polysulfate and glucosamine on the cytology, total protein concentration and viscosity of synovial fluid in horses

Kwan, C, Bell, RJ, Koenig, T, Bischofberger, A, Horadogoda, N, Perkins, NR, Jeffcott, LB and Dart, AJ (2012) Effects of intra-articular sodium pentosan polysulfate and glucosamine on the cytology, total protein concentration and viscosity of synovial fluid in horses. Australian Veterinary Journal, 90 8: 315-320. doi:10.1111/j.1751-0813.2012.00959.x


Author Kwan, C
Bell, RJ
Koenig, T
Bischofberger, A
Horadogoda, N
Perkins, NR
Jeffcott, LB
Dart, AJ
Title Effects of intra-articular sodium pentosan polysulfate and glucosamine on the cytology, total protein concentration and viscosity of synovial fluid in horses
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 2012-08
Year available 2012
Sub-type Article (original research)
DOI 10.1111/j.1751-0813.2012.00959.x
Open Access Status Not Open Access
Volume 90
Issue 8
Start page 315
End page 320
Total pages 6
Place of publication Chichester, West Sussex, United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective
Evaluate synovial fluid cytology, total protein concentration and viscosity after intra-articular administration of combined pentosan polysulfate and glucosamine in horses.

Design
Ten adult Standardbred horses had each carpal joint (n = 20) randomly assigned to one of two treatments: control (5 mL saline) or treated (pentosan polysulfate and glucosamine). All horses received an intra-articular injection every 7 days for 3 weeks and synovial fluid samples were collected on days 1 (baseline), 2, 3, 7, 8, 9, 14, 15, 16 and 21. Synovial fluid variables measured included total protein concentration, total nucleated cell count, red blood cell count, the neutrophil, lymphocyte, mononuclear cell and eosinophil percentages, and viscosity.

Results
Following injection, the total nucleated cell count increased in the synovial fluid samples from both groups. Values were significantly higher in treated joints on days 2, 3, 15 and 16. Red blood cell counts were low and no differences were seen between treated and control joints. Total protein concentrations and neutrophil percentages increased in both groups after injection, but decreased towards normal ranges within 7 days. Total protein concentration was significantly higher in treated joints on days 1, 3, 8, 9 and 15. Percentages of neutrophils were higher in treated joints on day 2, but significantly lower on days 8 and 15. Viscosity was significantly higher in treated joints on day 2 only.

Conclusions
Intra-articular administration of combined pentosan polysulfate and glucosamine causes a mild inflammatory synovitis that is not substantially different to that elicited by injection of a similar volume of saline and so we conclude that these drugs are safe to use in the horse.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Veterinary Science Publications
 
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Created: Wed, 30 Mar 2016, 17:59:02 EST by Nigel Perkins on behalf of School of Veterinary Science