Influence of interleukin-1 beta on platelet-poor plasma clot formation: a potential impact on early bone healing

Wang, Xin, Luo, Yan, Masci, Paul P., Crawford, Ross and Xiao, Yin (2016) Influence of interleukin-1 beta on platelet-poor plasma clot formation: a potential impact on early bone healing. Plos One, 11 2: . doi:10.1371/journal.pone.0149775


Author Wang, Xin
Luo, Yan
Masci, Paul P.
Crawford, Ross
Xiao, Yin
Title Influence of interleukin-1 beta on platelet-poor plasma clot formation: a potential impact on early bone healing
Journal name Plos One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-02-24
Year available 2016
Sub-type Article (original research)
DOI 10.1371/journal.pone.0149775
Open Access Status DOI
Volume 11
Issue 2
Total pages 13
Place of publication San Francisco, United States
Publisher Public Library of Science
Collection year 2017
Language eng
Formatted abstract
Objectives: Hematoma quality (especially the fibrin matrix) plays an important role in the bone healing process. Here, we investigated the effect of interleukin-1 beta (IL-1 beta) on fibrin clot formation from platelet-poor plasma (PPP).

Methods: Five-milliliter of rat whole-blood samples were collected from the hepatic portal vein. All blood samples were firstly standardized via a thrombelastograph (TEG), blood cell count, and the measurement of fibrinogen concentration. PPP was prepared by collecting the top two-fifths of the plasma after centrifugation under 400 x g for 10 min at 20 degrees C. The effects of IL-1 beta cytokines on artificial fibrin clot formation from PPP solutions were determined by scanning electronic microscopy (SEM), confocal microscopy (CM), turbidity, and clot lysis assays.

Results: The lag time for protofibril formation was markedly shortened in the IL-1 beta treatment groups (243.8 +/- 76.85 in the 50 pg/mL of IL-1 beta and 97.5 +/- 19.36 in the 500 pg/mL of IL-1 beta) compared to the control group without IL-1 beta (543.8 +/- 205.8). Maximal turbidity was observed in the control group. IL-1 beta (500 pg/mL) treatment significantly decreased fiber diameters resulting in smaller pore sizes and increased density of the fibrin clot structure formed from PPP (P < 0.05). The clot lysis assay revealed that 500 pg/mL IL-1 beta induced a lower susceptibility to dissolution due to the formation of thinner and denser fibers.

Conclusion: IL-1 beta can significantly influence PPP fibrin clot structure, which may affect the early bone healing process.
Keyword Platelet-rich plasma
PRP
Fracture Hematoma
Fibrin Structure
Polymerization
Regeneration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Admin Only - School of Medicine
School of Medicine Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Sun, 27 Mar 2016, 00:19:16 EST by System User on behalf of Learning and Research Services (UQ Library)