Molecular epidemiology of enterococcal bacteremia in Australia

Coombs, Geoffrey W., Pearson, Julie C., Daley, Denise A., Le, Tam, Robinson, Owen J., Gottlieb, Thomas, Howden, Benjamin P., Johnson, Paul D. R., Bennett, Catherine M., Stinear, Timothy P. and Turnidge, John D. (2014) Molecular epidemiology of enterococcal bacteremia in Australia. Journal of Clinical Microbiology, 52 3: 897-905. doi:10.1128/JCM.03286-13

Attached Files (Some files may be inaccessible until you login with your UQ eSpace credentials)
Name Description MIMEType Size Downloads
UQ383159_OA.pdf Full text (open access) application/pdf 1.52MB 0

Author Coombs, Geoffrey W.
Pearson, Julie C.
Daley, Denise A.
Le, Tam
Robinson, Owen J.
Gottlieb, Thomas
Howden, Benjamin P.
Johnson, Paul D. R.
Bennett, Catherine M.
Stinear, Timothy P.
Turnidge, John D.
Title Molecular epidemiology of enterococcal bacteremia in Australia
Journal name Journal of Clinical Microbiology   Check publisher's open access policy
ISSN 1098-660X
Publication date 2014-03
Sub-type Article (original research)
DOI 10.1128/JCM.03286-13
Open Access Status File (Publisher version)
Volume 52
Issue 3
Start page 897
End page 905
Total pages 9
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Enterococci are a major cause of health care-associated infections and account for approximately 10% of all bacteremias globally. The aim of this study was to determine the proportion of enterococcal bacteremia isolates in Australia that are antimicrobial resistant, with particular emphasis on susceptibility to ampicillin and the glycopeptides, and to characterize the molecular epidemiology of the Enterococcus faecalis and Enterococcus faecium isolates. From 1 January to 31 December 2011, 1,079 unique episodes of bacteremia were investigated, of which 95.8% were caused by either E. faecalis (61.0%) or E. faecium (34.8%). The majority of bacteremias were health care associated, and approximately one-third were polymicrobial. Ampicillin resistance was detected in 90.4% of E. faecium isolates but was not detected in E. faecalis isolates. Vancomycin nonsusceptibility was reported in 0.6% and 36.5% of E. faecalis and E. faecium isolates, respectively. Unlike Europe and the United States, where vancomycin resistance in E. faecium is predominately due to the acquisition of the vanA operon, 98.4% of E. faecium isolates harboring van genes carried the vanB operon, and 16.1% of the vanB E. faecium isolates had vancomycin MICs at or below the susceptible breakpoint of the CLSI. Although molecular typing identified 126 E. faecalis pulsed-field gel electrophoresis pulsotypes, >50% belonged to two pulsotypes that were isolated across Australia. E. faecium consisted of 73 pulsotypes from which 43 multilocus sequence types were identified. Almost 90% of the E. faecium isolates were identified as CC17 clones, of which approximately half were characterized as ST203, which was isolated Australia-wide. In conclusion, the Australian Enterococcal Sepsis Outcome Programme (AESOP) study has shown that although they are polyclonal, enterococcal bacteremias in Australia are frequently caused by ampicillin-resistant vanB E. faecium
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Teaching and Learning Innovation Publications
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 7 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 9 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 25 Mar 2016, 18:46:29 EST by System User on behalf of Learning and Research Services (UQ Library)