Characterisation and cross-amplification of 19 novel microsatellite loci for the sandbar shark, Carcharhinus plumbeus

Blower, D. C., Corley, S., Hereward, J. P., Riginos, C. R. and Ovenden, J. R. (2015) Characterisation and cross-amplification of 19 novel microsatellite loci for the sandbar shark, Carcharhinus plumbeus. Conservation Genetics Resources, 7 4: 913-915. doi:10.1007/s12686-015-0500-0


Author Blower, D. C.
Corley, S.
Hereward, J. P.
Riginos, C. R.
Ovenden, J. R.
Title Characterisation and cross-amplification of 19 novel microsatellite loci for the sandbar shark, Carcharhinus plumbeus
Formatted title
Characterisation and cross-amplification of 19 novel microsatellite loci for the sandbar shark, Carcharhinus plumbeus
Journal name Conservation Genetics Resources   Check publisher's open access policy
ISSN 1877-7252
1877-7260
Publication date 2015-12-01
Sub-type Article (original research)
DOI 10.1007/s12686-015-0500-0
Open Access Status Not Open Access
Volume 7
Issue 4
Start page 913
End page 915
Total pages 3
Place of publication Dordrecht, Netherlands
Publisher Springer Netherlands
Collection year 2016
Language eng
Formatted abstract
Conservation of vulnerable shark species that are also heavily exploited, such as the sandbar shark Carcharhinus plumbeus is a pressing issue, and molecular genetics is an increasingly important population monitoring and species identification tool. Here we present 19 C. plumbeus microsatellite loci with a broad range of alleles per locus (3–22), and observed heterozygosities (0.17–0.95). Null alleles were not apparent and if present, were at frequencies <0.041. None of the loci showed significant deviation from Hardy–Weinberg equilibrium or indications of linkage disequilibrium. Cross-species amplification of these loci in three other carcharinids, dusky shark Carcharhinus obscurus, spinner shark Carcharhinus brevipinna, and galapagos shark Carcharhinus galapagensis showed polymorphism in 13, 13, and 14 loci respectively. These highly diverse loci will assist both individual-specific and population-wide genetic investigations for these species.
Keyword Carcharhinus plumbeus
Sandbar shark
Microsatellite
454 sequencing
Cross-amplification
Q-Index Code CX
Q-Index Status Provisional Code
Institutional Status UQ

 
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