Sexing pinnipeds with ZFX and ZFY loci

Curtis, Caitlin, Stewart, Brent S. and Karl, Stephen A. (2007) Sexing pinnipeds with ZFX and ZFY loci. Journal of Heredity, 98 3: 280-285. doi:10.1093/jhered/esm023

Author Curtis, Caitlin
Stewart, Brent S.
Karl, Stephen A.
Title Sexing pinnipeds with ZFX and ZFY loci
Journal name Journal of Heredity   Check publisher's open access policy
ISSN 0022-1503
Publication date 2007
Sub-type Article (original research)
DOI 10.1093/jhered/esm023
Volume 98
Issue 3
Start page 280
End page 285
Total pages 6
Language eng
Subject 1100 Agricultural and Biological Sciences
1311 Genetics
2716 Genetics (clinical)
Abstract We developed and tested a protocol for determining the sex of individual pinnipeds using the sex-chromosome-specific genes ZFX and ZFY. We screened a total of 368 seals (168 crabeater, Lobodon carcinophaga; 159 Weddell, Leptonychotes weddellii; and 41 Ross, Ommatophoca rossii) of known or unknown sex and compared the molecular sex to the sex assigned at the time of biopsy sample collection in the Ross and Amundsen seas, Antarctica. We also screened 6 captive northern elephant seals (Mirounga angustirostris) and 2 captive California sea lions (Zalophus californianus) of known sex. The assigned sex and genetic sex agreed for virtually all seals. Indeed, discrepancies ranged from 0.0% to 6.7% among species. It is not clear, however, if the few mis-assignments of sex occurred in situ or in the laboratory. The assigned morphological and molecular sex might both be correct with the discrepancies owing perhaps to developmental effects of environmental pollution. A subset of individuals sequenced at both loci revealed no intraspecific sequence variation. There was, however, sequence variation among species at both loci, which allowed them to be uniquely identified with as few as 2 and as many as 31 nucleotides.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import
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Citation counts: TR Web of Science Citation Count  Cited 10 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 13 times in Scopus Article | Citations
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Created: Wed, 23 Mar 2016, 15:26:52 EST by Caitlin Curtis