X-ray diffraction images related to PDB ID 5EM9

Collins, Brett (2016): X-ray diffraction images related to PDB ID 5EM9. The University of Queensland. Dataset. doi:10.14264/uql.2016.116

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Related Publications and Datasets
Project name Recruitment of PDZ cargo proteins by the SNX27-retromer complex is promoted by cargo phosphorylation
Project description
Recycling of internalised receptors from endosomal compartments is essential for their cell surface homeostasis. Sorting nexin 27 (SNX27) cooperates with the retromer complex in the recycling of proteins containing type I PSD95/Dlg/ZO1 (PDZ) binding motifs. Here we define specific acidic amino acid sequences upstream of the PDZ motif required for high affinity engagement of the SNX27 PDZ domain. However, a subset of SNX27 ligands such as the β2-adrenergic and N-methyl-D-aspartate (NMDA) receptors lack these sequence determinants. Instead we identify conserved sites of phosphorylation that substitute for acidic residues to dramatically enhance SNX27 interactions. This newly identified mechanism points to a likely regulatory switch for PDZ interaction and protein transport by SNX27-retromer. Defining this SNX27-binding code has allowed us to classify more than four hundred potential SNX27 ligands with broad functional implications in signal transduction, neuronal plasticity and metabolite transport.
Contact name Brett Collins
Contact email b.collins@imb.uq.edu.au
Creator name Collins, Brett
Creator(s) role Chief Investigator
Dataset name X-ray diffraction images related to PDB ID 5EM9
Dataset description
X-ray diffraction Images collected at the Australian Synchrotron.
Access conditions Open Access
Licencing and terms of access Creative Commons Attribution (only)

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ANZSRC Field of Research (FoR) Code 0601 Biochemistry and Cell Biology
DOI 10.14264/uql.2016.116
Related Publications
Grant ID APP1042082, APP1058734, APP1078280, DP0985029, APP1061574
Type of data 180 X-ray diffraction images
Data Volume 1.3 GB
Software required XDS, mosflm or other image viewer
Language eng
Collection start date 2016-03-01
Collection end date 2016-03-01
Keyword X-ray crystallography
PDZ binding motif
ANZSRC Socio-Economic Objective (SEO) Code 970106 Expanding Knowledge in the Biological Sciences
Collection type Dataset
Publisher The University of Queensland
Publication Year 2016
Copyright notice The University of Queensland

Document type: Data Collection
Collections: Queensland Brain Institute Publications
Research Data Collections
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Created: Wed, 23 Mar 2016, 12:55:44 EST by Brett Collins