Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia

Sinclair, Holly A., Heney, Claire, Sidjabat, Hanna E., George, Narelle M., Bergh, Haakon, Anuj, Snehal N., Nimmo, Graeme R. and Paterson, David L. (2016) Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia. Diagnostic Microbiology and Infectious Disease, 85 1: 98-101. doi:10.1016/j.diagmicrobio.2016.02.005


Author Sinclair, Holly A.
Heney, Claire
Sidjabat, Hanna E.
George, Narelle M.
Bergh, Haakon
Anuj, Snehal N.
Nimmo, Graeme R.
Paterson, David L.
Title Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia
Formatted title
Genotypic and phenotypic identification of Aeromonas species and CphA-mediated carbapenem resistance in Queensland, Australia
Journal name Diagnostic Microbiology and Infectious Disease   Check publisher's open access policy
ISSN 1879-0070
0732-8893
Publication date 2016-05
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.diagmicrobio.2016.02.005
Open Access Status Not Open Access
Volume 85
Issue 1
Start page 98
End page 101
Total pages 4
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Infection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene, is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, Australia. Phenotypic tests for carbapenemase detection were assessed. One hundred Aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by rpoB and gyrB sequencing. Meropenem susceptibility by VITEK2, disk diffusion, and E-test MIC were determined. Carbapenemase production was assessed by Carba NP test and cphA by PCR. Gene sequencing identified isolates as Aeromonas dhakensis (39), Aeromonas veronii (21), Aeromonas hydrophila (20), Aeromonas caviae (14), Aeromonas jandaei (4), Aeromonas bestiarum (1), and Aeromonas sanarellii (1). Disk diffusion and E-test failed to detect resistance in isolates with presence of cphA. Carba NP was performed with 97.4% sensitivity and 95.7% specificity. Carbapenem resistance gene cphA was detected in A. veronii (21; 100%), A. hydrophila (18; 90%), A. dhakensis (34; 87.2%), A. jandaei (3; 75%), and A. bestiarum (1; 100%) but not A. caviae. We found that A. dhakensis was the predominant species, a previously unrecognized pathogen in this region.
Keyword Aeromonas
CphA
Carbapenem resistance
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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