Bilaterian-like promoters in the highly compact Amphimedon queenslandica genome

Fernandez-Valverde, Selene L. and Degnan, Bernard M. (2016) Bilaterian-like promoters in the highly compact Amphimedon queenslandica genome. Scientific Reports, 6 Art No.: 22496: . doi:10.1038/srep22496

Author Fernandez-Valverde, Selene L.
Degnan, Bernard M.
Title Bilaterian-like promoters in the highly compact Amphimedon queenslandica genome
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-03-02
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep22496
Open Access Status DOI
Volume 6
Issue Art No.: 22496
Total pages 11
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Subject 1000 General
Formatted abstract
The regulatory systems underlying animal development must have evolved prior to the emergence of eumetazoans (cnidarians and bilaterians). Although representatives of earlier-branching animals – sponges ctenophores and placozoans – possess most of the developmental transcription factor families present in eumetazoans, the DNA regulatory elements that these transcription factors target remain uncharted. Here we characterise the core promoter sequences, U1 snRNP-binding sites (5′ splice sites; 5′SSs) and polyadenylation sites (PASs) in the sponge Amphimedon queenslandica. Similar to unicellular opisthokonts, Amphimedon’s genes are tightly packed in the genome and have small introns. In contrast, its genes possess metazoan-like core promoters populated with binding motifs previously deemed to be specific to vertebrates, including Nrf-1 and Krüppel-like elements. Also as in vertebrates, Amphimedon’s PASs and 5′SSs are depleted downstream and upstream of transcription start sites, respectively, consistent with non-elongating transcripts being short-lived; PASs and 5′SSs are more evenly distributed in bidirectional promoters in Amphimedon. The presence of bilaterian-like regulatory DNAs in sponges is consistent with these being early and essential innovations of the metazoan gene regulatory repertoire.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
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