Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep

Richa, Kamboj, Tiwari, Ila M., Kumari, Mandeep, Devanna, B. N., Sonah, Humira, Kumari, Archana, Nagar, Ramawatar, Sharma, Vinay, Botella, Jose R. and Sharma, Tilak R. (2016) Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep. Frontiers in Plant Science, 7 244: 1-10. doi:10.3389/fpls.2016.00244


Author Richa, Kamboj
Tiwari, Ila M.
Kumari, Mandeep
Devanna, B. N.
Sonah, Humira
Kumari, Archana
Nagar, Ramawatar
Sharma, Vinay
Botella, Jose R.
Sharma, Tilak R.
Title Functional characterization of novel chitinase genes present in the sheath blight resistance QTL: Qsbr11-1 in rice line Tetep
Journal name Frontiers in Plant Science   Check publisher's open access policy
ISSN 1664-462X
Publication date 2016-03-01
Year available 2016
Sub-type Article (original research)
DOI 10.3389/fpls.2016.00244
Open Access Status DOI
Volume 7
Issue 244
Start page 1
End page 10
Total pages 10
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Collection year 2017
Language eng
Formatted abstract
Rice sheath blight disease caused by Rhizoctonia solani is one of the most devastating diseases in rice leading to heavy yield losses. Due to the polygenic nature of resistance, no major resistance gene with complete host resistance against R. solani has been reported. In this study, we have performed molecular and functional analysis of the genes associated with the major R. solani-resistance QTL qSBR11-1 in the indica rice line Tetep. Sequence analysis revealed the presence of a set of 11 tandem repeats containing genes with a high degree of homology to class III chitinase defense response genes. Real-time quantitative PCR analysis showed that all the genes are strongly induced 36 h after R. solani infection. Comparison between the resistant Tetep and the susceptible HP2216 lines shows that the induction of the chitinase genes is much higher in the Tetep line. Recombinant protein produced in vitro for six of the eleven genes showed chitinolytic activity in gel assays but we did not detect any xylanase inhibitory activity. All the six in vitro expressed proteins show antifungal activity with a clear inhibitory effect on the growth of the R. solani mycelium. The characterized chitinase genes can provide an important resource for the genetic improvement of R. solani susceptible rice lines for sheath blight resistance breeding.
Keyword Chitinase
Rhizoctonia solani
Sheath blight
Tetep
Xylanase
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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