A generic screening platform for inhibitors of virus induced cell fusion using cellular electrical impedance

Watterson, Daniel, Robinson, Jodie, Chappell, Keith J., Butler, Mark S., Edwards, David J., Fry, Scott R., Bermingham, Imogen M., Cooper, Matthew A. and Young, Paul R. (2016) A generic screening platform for inhibitors of virus induced cell fusion using cellular electrical impedance. Scientific Reports, 6 22791: 1-9. doi:10.1038/srep22791


Author Watterson, Daniel
Robinson, Jodie
Chappell, Keith J.
Butler, Mark S.
Edwards, David J.
Fry, Scott R.
Bermingham, Imogen M.
Cooper, Matthew A.
Young, Paul R.
Title A generic screening platform for inhibitors of virus induced cell fusion using cellular electrical impedance
Journal name Scientific Reports   Check publisher's open access policy
ISSN 2045-2322
Publication date 2016-03-15
Year available 2016
Sub-type Article (original research)
DOI 10.1038/srep22791
Open Access Status DOI
Volume 6
Issue 22791
Start page 1
End page 9
Total pages 9
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Collection year 2017
Language eng
Abstract Fusion of the viral envelope with host cell membranes is an essential step in the life cycle of all enveloped viruses. Despite such a clear target for antiviral drug development, few anti-fusion drugs have progressed to market. One significant hurdle is the absence of a generic, high-throughput, reproducible fusion assay. Here we report that real time, label-free measurement of cellular electrical impedance can quantify cell-cell fusion mediated by either individually expressed recombinant viral fusion proteins, or native virus infection. We validated this approach for all three classes of viral fusion and demonstrated utility in quantifying fusion inhibition using antibodies and small molecule inhibitors specific for dengue virus and respiratory syncytial virus.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
Institute for Molecular Bioscience - Publications
 
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Created: Fri, 18 Mar 2016, 11:50:58 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences