Protective efficacy afforded by live Pasteurella multocida vaccines in chickens is independent of lipopolysaccharide outer core structure

Harper, Marina, John, Marietta, Edmunds, Mark, Wright, Amy, Ford, Mark, Turni, Conny, Blackall, P. J., Cox, Andrew, Adler, Ben and Boyce, John D. (2016) Protective efficacy afforded by live Pasteurella multocida vaccines in chickens is independent of lipopolysaccharide outer core structure. Vaccine, 34 14: 1696-1703. doi:10.1016/j.vaccine.2016.02.017


Author Harper, Marina
John, Marietta
Edmunds, Mark
Wright, Amy
Ford, Mark
Turni, Conny
Blackall, P. J.
Cox, Andrew
Adler, Ben
Boyce, John D.
Title Protective efficacy afforded by live Pasteurella multocida vaccines in chickens is independent of lipopolysaccharide outer core structure
Formatted title
Protective efficacy afforded by live Pasteurella multocida vaccines in chickens is independent of lipopolysaccharide outer core structure
Journal name Vaccine   Check publisher's open access policy
ISSN 1873-2518
0264-410X
Publication date 2016-03-29
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.vaccine.2016.02.017
Open Access Status Not Open Access
Volume 34
Issue 14
Start page 1696
End page 1703
Total pages 8
Place of publication London, United Kingdom
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Pasteurella multocida is a major animal pathogen that causes a range of diseases including fowl cholera. P. multocida infections result in considerable losses to layer and breeder flocks in poultry industries worldwide. Both killed whole-cell and live-attenuated vaccines are available; these vaccines vary in their protective efficacy, particularly against heterologous strains. Moreover, until recently there was no knowledge of P. multocida LPS genetics and structure to determine precisely how LPS structure affects the protective capacity of these vaccines. In this study we show that defined lipopolysaccharide (LPS) mutants presented as killed whole-cell vaccines elicited solid protective immunity only against P. multocida challenge strains expressing highly similar or identical LPS structures. This finding indicates that vaccination of commercial flocks with P. multocida killed cell formulations will not protect against strains producing an LPS structure different to that produced by strains included in the vaccine formulation. Conversely, protective immunity conferred by vaccination with live P. multocida strains was found to be largely independent of LPS structure. Birds vaccinated with a range of live mutants belonging to the L1 and L3 LPS genotypes, each expressing a specific truncated LPS structure, were protected against challenge with the parent strain. Moreover, birds vaccinated with any of the five LPS mutants belonging to the L1 LPS genotype were also protected against challenge with an unrelated strain and two of the five groups vaccinated with live LPS mutants belonging to the L3 genotype were protected against challenge with an unrelated strain. In summary, vaccination with live P. multocida aroA mutants producing full-length L1 or L3 LPS or vaccination with live strains producing shortened L1 LPS elicited strong protective immunity against both homologous and heterologous challenge.
Keyword Pasteurella multocida
Vaccine
Lipopolysaccharide
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
 
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