Mobilization of hematopoietic stem cells with highest self-renewal by G-CSF precedes clonogenic cell mobilization peak

Winkler, Ingrid G., Wiercinska, Eliza, Barbier, Valerie, Nowlan, Bianca, Bonig, Halvard and Levesque, Jean-Pierre (2016) Mobilization of hematopoietic stem cells with highest self-renewal by G-CSF precedes clonogenic cell mobilization peak. Experimental Hematology, 44 4: 303-314.e1. doi:10.1016/j.exphem.2016.01.001


Author Winkler, Ingrid G.
Wiercinska, Eliza
Barbier, Valerie
Nowlan, Bianca
Bonig, Halvard
Levesque, Jean-Pierre
Title Mobilization of hematopoietic stem cells with highest self-renewal by G-CSF precedes clonogenic cell mobilization peak
Journal name Experimental Hematology   Check publisher's open access policy
ISSN 1873-2399
0301-472X
Publication date 2016-04
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.exphem.2016.01.001
Open Access Status Not Open Access
Volume 44
Issue 4
Start page 303
End page 314.e1
Total pages 13
Place of publication Philadelphia, PA United States
Publisher Elsevier
Collection year 2017
Language eng
Formatted abstract
Harvest of granulocyte colony-stimulating factor (G-CSF)-mobilized hematopoietic stem cells (HSCs) begins at day 5 of G-CSF administration, when most donors have achieved maximal mobilization. This is based on surrogate markers for HSC mobilization, such as CD34+ cells and colony-forming activity in blood. However, CD34+ cells or colony-forming units in culture (CFU-C) are heterogeneous cell populations with hugely divergent long-term repopulation potential on transplantation. HSC behavior is influenced by the vascular bed in the vicinity of which they reside. We hypothesized that G-CSF may mobilize sequentially cells proximal and more distal to bone marrow venous sinuses where HSCs enter the blood. We addressed this question with functional serial transplantation assays using blood and bone marrow after specific time points of G-CSF treatment in mice. We found that in mice, blood collected after only 48 hours of G-CSF administration was as enriched in serially reconstituting HSCs as blood collected at 5 days of G-CSF treatment. Similarly, mobilized LinCD34+ cells were relatively enriched in more primitive LinCD34+CD38 cells at day 2 of G-CSF treatment compared with later points in half of human donors tested (n = 6). This suggests that in both humans and mice, hematopoietic progenitor and stem cells do not mobilize uniformly according to their maturation stage, with most potent HSCs mobilizing as early as day 2 of G-CSF.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
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