Molecular approaches to trematode systematics: ‘best practice’ and implications for future study

Blasco-Costa, Isabel, Cutmore, Scott .C., Miller, Terrence L. and Nolan, Matthew J. (2016) Molecular approaches to trematode systematics: ‘best practice’ and implications for future study. Systematic Parasitology, 93 3: 295-306. doi:10.1007/s11230-016-9631-2


Author Blasco-Costa, Isabel
Cutmore, Scott .C.
Miller, Terrence L.
Nolan, Matthew J.
Title Molecular approaches to trematode systematics: ‘best practice’ and implications for future study
Journal name Systematic Parasitology   Check publisher's open access policy
ISSN 1573-5192
0165-5752
Publication date 2016-03-01
Year available 2016
Sub-type Article (original research)
DOI 10.1007/s11230-016-9631-2
Open Access Status Not Open Access
Volume 93
Issue 3
Start page 295
End page 306
Total pages 12
Place of publication Dordrecht, The Netherlands
Publisher Springer Netherlands
Collection year 2017
Language eng
Subject 2405 Parasitology
Formatted abstract
To date, morphological analysis has been the cornerstone to trematode systematics. However, since the late-1980s we have seen an increased integration of genetic data to overcome problems encountered when morphological data are considered in isolation. Here, we provide advice regarding the ‘best molecular practice’ for trematode taxonomy and systematic studies, in an attempt to help unify the field and provide a solid foundation to underpin future work. Emphasis is placed on defining the study goals and recommendations are made regarding sample preservation, extraction methods, and the submission of molecular vouchers. We advocate generating sequence data from all parasite species/host species/geographic location combinations and stress the importance of selecting two independently evolving loci (one ribosomal and one mitochondrial marker). We recommend that loci should be chosen to provide genetic variation suitable to address the question at hand and for which sufficient ‘useful’ comparative sequence data already exist. Quality control of the molecular data via using proof-reading Taq polymerase, sequencing PCR amplicons using both forward and reverse primers, ensuring that a minimum of 85% overlap exists when constructing consensus sequences, and checking electropherograms by eye is stressed. We advise that all genetic results are best interpreted using a holistic biological approach, which considers morphology, host identity, collection locality, and ecology. Finally, we consider what advances next-generation sequencing holds for trematode taxonomy and systematics.
Special Issue on Biodiversity of Tremato
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biological Sciences Publications
 
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